智能手机辅助双探针比色法检测α-葡萄糖苷酶OA北大核心CSTPCD
Detection of α-Glucosidase by a Smartphone Assisted Dual-probe Colorimetric Method
该文以3,3',5,5'-四甲基联苯胺(TMB)和罗丹明B为比色探针,建立了α-葡萄糖苷酶的比色检测方法,并在此基础上基于智能手机策略,通过RGB值对α-葡萄糖苷酶进行检测.结果表明,Fe3+-TMB-罗丹明B显色体系的吸光度与α-葡萄糖苷浓度在20～100 U/L范围内呈线性关系,检出限为3.3 U/L.采用photo-shop软件分析样品图像和样品荧光图像的RGB值,其与α-葡萄糖苷酶浓度在10～100 U/L范围内呈线性关系,检出限分别为2.5 U/L和1.2 U/L.该法已成功用于α-葡萄糖苷酶抑制剂阿卡波糖抑制率评估和胎牛血清中α-葡萄糖苷酶浓度的测定.所构建的智能手机辅助检测法为α-葡萄糖苷酶的临床分析和酶抑制剂的快速筛选提供了简便、快捷和准确的方法.
This study exploited 3,3',5,5'-tetramethylbenzidine(TMB)and Rhodamine B as chromogenic probes for α-glucosidase concentration analysis via a colorimetric detection method.On this basis,a smartphone assisted strategy was designed to detect α-glucosidase activity by RGB value analysis of images.The results showed that the absorbance of Fe3+-TMB-Rhodamine B system was linearly related to α-glucosidase concentration in the range of 20-100 U/L,and the detection limit was 3.3 U/L.The RGB values of the sample photos and fluorescence photos were analyzed by photo-shop,which showed a linear relationship with α-glucosidase activity in the range of 10-100 U/L,the detection limits were 2.5 U/L and 1.2 U/L for α-glucosidase,respectively.The method has been successfully used to detect α-glucosidase inhibitor acarbose and to quantitatively detect α-glucosidase activity in spiked fetal bovine serum.The smartphone assisted detection method provided a simple,quick and accurate method for clinical analysis of α-glucosidase and rapid screening of enzyme inhibi-tors.
赵灵芝;赵柳;张小清;寇张文;魏世楠
西安医学院 药学院,陕西 西安 710021||陕西师范大学 化学化工学院 陕西省生命分析重点实验室,陕西 西安 710062陕西省农业检验检测中心,陕西 西安 710003西安医学院 药学院,陕西 西安 710021
化学
α-葡萄糖苷酶抗坏血酸智能手机比色法3,3',5,5'-四甲基联苯胺(TMB)罗丹明 B
α-glucosidaseascorbic acidsmartphone assisted colorimetry3,3',5,5'-tetra-methylbenzidine(TMB)Rhodamine B
《分析测试学报》 2024 (006)
905-912 / 8
陕西省科技厅项目(2022JM-094,2021JM-502);西安医学院杰出人才青年计划配套项目(2021PT08);2022年科研能力提升计划项目(2022NLTS072);陕西省科技厅社发项目(2024SF-YBXM-661);咸阳市科技局社发项目(L2023-ZDYF-SF-023)
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