分析测试学报2024，Vol.43Issue(6)：905-912,8.DOI:10.12452/j.fxcsxb.23122701
智能手机辅助双探针比色法检测α-葡萄糖苷酶
Detection of α-Glucosidase by a Smartphone Assisted Dual-probe Colorimetric Method
摘要
Abstract
This study exploited 3,3',5,5'-tetramethylbenzidine(TMB)and Rhodamine B as chromogenic probes for α-glucosidase concentration analysis via a colorimetric detection method.On this basis,a smartphone assisted strategy was designed to detect α-glucosidase activity by RGB value analysis of images.The results showed that the absorbance of Fe3+-TMB-Rhodamine B system was linearly related to α-glucosidase concentration in the range of 20-100 U/L,and the detection limit was 3.3 U/L.The RGB values of the sample photos and fluorescence photos were analyzed by photo-shop,which showed a linear relationship with α-glucosidase activity in the range of 10-100 U/L,the detection limits were 2.5 U/L and 1.2 U/L for α-glucosidase,respectively.The method has been successfully used to detect α-glucosidase inhibitor acarbose and to quantitatively detect α-glucosidase activity in spiked fetal bovine serum.The smartphone assisted detection method provided a simple,quick and accurate method for clinical analysis of α-glucosidase and rapid screening of enzyme inhibi-tors.关键词
α-葡萄糖苷酶/抗坏血酸/智能手机比色法/3,3',5,5'-四甲基联苯胺(TMB)/罗丹明 BKey words
α-glucosidase/ascorbic acid/smartphone assisted colorimetry/3,3',5,5'-tetra-methylbenzidine(TMB)/Rhodamine B分类
化学化工引用本文复制引用
赵灵芝,赵柳,张小清,寇张文,魏世楠..智能手机辅助双探针比色法检测α-葡萄糖苷酶[J].分析测试学报,2024,43(6):905-912,8.基金项目
陕西省科技厅项目(2022JM-094,2021JM-502) （2022JM-094,2021JM-502）
西安医学院杰出人才青年计划配套项目(2021PT08) （2021PT08）
2022年科研能力提升计划项目(2022NLTS072) （2022NLTS072）
陕西省科技厅社发项目(2024SF-YBXM-661) （2024SF-YBXM-661）
咸阳市科技局社发项目(L2023-ZDYF-SF-023) （L2023-ZDYF-SF-023）
