免疫磁珠净化/高效液相色谱检测植物油中黄曲霉毒素含量OA北大核心CSTPCD

In this study,a high performance liquid chromatography method based on immunomag-netic beads purification for the determination of aflatoxin B1(AFB1),aflatoxin B2(AFB2),aflatoxin G1(AFG1)and aflatoxin G2(AFG2))in vegetable oils was established.First,the aflatoxins in the sample were extracted with methanol-water(70∶30,volume ratio).The samples were purified with aflatoxin total immunomagnetic bead kit,combined with the immunomagnetic bead automatic work-station,which significantly improved the purification efficiency.Aflatoxins showed good linearity with correlation coefficients(r2)more than 0.99.The limits of quantitation(LOQs)for AFB1 and AFB2,AFG1 and AFG2 were 0.05 μg/kg and 0.10 μg/kg,respectively.The average recoveries of af-latoxins in five commercial vegetable oils ranged from 74.0%to 96.0%,with the intra-day and intra-day relative standard deviations(RSDs)less than 7.0%.The results of the comparison experiments showed that the method had the same performance as the national standard GB 5009.22-2016.The established method has good accuracy and sensitivity,which is suitable for simultaneous quantitative detection of four aflatoxins in vegetable oils.