分析测试学报2024，Vol.43Issue(6)：928-932,5.DOI:10.12452/j.fxcsxb.24012901
基于PCR-LFIA的非洲猪瘟病毒核酸检测方法研究
Study on Nucleic Acid Detection Method of African Swine Fever Vi-rus Based on PCR-LFIA
摘要
Abstract
In this paper,polymerase chain reaction(PCR)was used to amplify nucleic acid,lateral flow immunoassay(LFIA)was used to detect nucleic acid,and a rapid detection method for African swine fever virus nucleic acid was established.In this study,by designing primers,the positive plasmid pUC57-ASFV-(1-1941)was constructed as a quality control product and colloidal gold par-ticles were prepared by trisodium citrate reduction method for antibody labeling to establish an immu-noassay method.Under the optimal experimental conditions,the plasmid concentration was in the range of 1.6×10-2-1.6×108 copies/μL,the detection limit of pUC57-ASFV-(1-1941)positive plas-mid was 1.6 copies/μL,the method had no cross with other pig viruses and had good specificity.The established method can be used as an auxiliary method for field screening of African swine fever.关键词
非洲猪瘟/聚合酶链式反应(PCR)/免疫层析/可视化Key words
African swine fever/polymerase chain reaction(PCR)/immunochromatography/vi-sualization分类
化学化工引用本文复制引用
向四意,苏晓娜,张咏仪,陈俊杰,杨旭琼,沈玉栋,杨金易..基于PCR-LFIA的非洲猪瘟病毒核酸检测方法研究[J].分析测试学报,2024,43(6):928-932,5.基金项目
新型生物快速检测技术研究及应用开发(h20230751) （h20230751）
