聚乳酸改性糯玉米淀粉酶解产物的结构解析OA北大核心CSTPCD

Using polylactic acid modified waxy corn starch as raw material,the effects of different amylases on reducing sugar content of polylactic acid modified waxy corn starch were investigated,and the optimal enzymolysis method was obtained:isoamylase hydrolysis for 10 h,pullulanase hy-drolysis for 10 h,α-amylase hydrolysis for 10 h,and β-amylase hydrolysis for 12 h.The enzymoly-sis products were extracted with cyclohexane,the results of 1 H NMR showed that carbohydrates con-taining lactic acid groups could be effectively extracted and enriched,and separated from unsubstitut-ed carbohydrates;Mass spectrometry analysis showed that polylactic acid modified waxy corn starch was mainly composed of lactic acid ester and dipolylactic acid ester.This study explored and estab-lished an effective separation and analysis method,and promoted the scientific understanding of the fine structure of starch esters.