分析测试学报2024，Vol.43Issue(6)：933-938,6.DOI:10.12452/j.fxcsxb.23121962
聚乳酸改性糯玉米淀粉酶解产物的结构解析
Analysis for Enzymatic Hydrolysis Products of Polylactic Acid Modi-fied Waxy Corn Starch
摘要
Abstract
Using polylactic acid modified waxy corn starch as raw material,the effects of different amylases on reducing sugar content of polylactic acid modified waxy corn starch were investigated,and the optimal enzymolysis method was obtained:isoamylase hydrolysis for 10 h,pullulanase hy-drolysis for 10 h,α-amylase hydrolysis for 10 h,and β-amylase hydrolysis for 12 h.The enzymoly-sis products were extracted with cyclohexane,the results of 1 H NMR showed that carbohydrates con-taining lactic acid groups could be effectively extracted and enriched,and separated from unsubstitut-ed carbohydrates;Mass spectrometry analysis showed that polylactic acid modified waxy corn starch was mainly composed of lactic acid ester and dipolylactic acid ester.This study explored and estab-lished an effective separation and analysis method,and promoted the scientific understanding of the fine structure of starch esters.关键词
聚乳酸改性糯玉米淀粉/酶解/还原糖含量/环己烷提取/乳酸酯Key words
polylactic acid modified waxy corn starch/enzymatic hydrolysis/reducing sugar con-tent/cyclohexane extraction/lactate ester分类
化学化工引用本文复制引用
任逸,周坤,何宇鹏,姚利辉,仇丹..聚乳酸改性糯玉米淀粉酶解产物的结构解析[J].分析测试学报,2024,43(6):933-938,6.基金项目
宁波市自然科学基金(2021J142,2022J003) （2021J142,2022J003）
国家自然科学基金(21978124) （21978124）
镇海区"十四五"技术攻关重大专项计划 （）
