分析测试学报2024，Vol.43Issue(6)：939-944,6.DOI:10.12452/j.fxcsxb.24010801
液相色谱-串联质谱法测定干血点中晕车药及代谢物
Determination of Motion Sickness Drugs and Metabolite in Dried Blood Spots by Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
In this paper,a liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was developed for the determination of scopolamine,atropine,diphenidol,theophylline,diphen-hydramine and benzophenone in dried blood spots(DBS).A dried blood spot was prepared by taking 20 μL of blood,extracted by methanol-acetonitrile(8∶2,volume ratio)as solvent by ultrasonica-tion,separated on a Thermo Gold ODS(150 mm×2.1 mm,5 μm)column,with methanol-5 mmol/L ammonium acetate solution(containing 0.2%glacial acetic acid,pH 4.0)was used as the mobile phase for gradient elution,and then measured in multiple reaction monitoring(MRM)mode of the positive-ion mode,and quantified by external standard method.The results showed that the linearity of the six targets in dried blood spots was good within a certain mass concentration range,and the cor-relation coefficients(r2)were not less than 0.993 0.The limits of detection(LODs)ranged from 3 to 30 ng/mL,and the limits of quantification(LOQs)ranged from 10 to 100 ng/mL.The average recov-eries of the six targets ranged from 88.8%to 108%at low,medium and high concentration levels,with the inter-day and intra-day relative standard deviations were less than 15%.The method was sim-ple and fast,with high accuracy and sensitivity,and has been successfully applied to the detection of one case of difenidol poisoning,which provides a new method for the determination of cases of mo-tion sickness drug poisoning.关键词
干血点/晕车药/代谢物/液相色谱-串联质谱法Key words
dried blood spot/motion sickness drugs/metabolite/liquid chromatography-tandem mass spectrometry分类
化学化工引用本文复制引用
吴浩天,陈学国,田露,杜政达..液相色谱-串联质谱法测定干血点中晕车药及代谢物[J].分析测试学报,2024,43(6):939-944,6.基金项目
法庭毒物分析公安部重点实验室开放课题(2023FTDWFX03) （2023FTDWFX03）
2023年度中国刑事警察学院中央高校基本科研业务费项目(C2023009) （C2023009）
2023年度教育厅基本科研项目(JYTMS20231413) （JYTMS20231413）
2023年度研究生创新能力提升项目(2023YCYB51) （2023YCYB51）
公安部科技计划项目(2023JSYJC22) （2023JSYJC22）
