分析化学2024，Vol.52Issue(5)：609-623,15.DOI:10.19756/j.issn.0253-3820.231219
磷酸化蛋白质的定量与化学计量分析方法的研究进展
Advances in Quantification and Site Stoichiometry Analysis Methods for Phosphorylated Proteins
摘要
Abstract
The post-translational modification of proteins is a key mechanism that imparts physiological functions to proteins,among which reversible phosphorylation modifications play a pivotal role in many biological processes.Aberrant changes in phosphorylation are often closely associated with various major disease processes.In recent years,with the aid of proteomic technologies and methods,high-throughput,high-precision qualitative and quantitative approaches for phosphorylated proteins have rapidly advanced.This article reviews the research progress of phosphorylated protein quantification and chemical proteomics analysis methods based on the"bottom-up"strategy,including phosphopeptide enrichment methods,mass spectrometry fragmentation methods,quantification analysis methods and phosphorylation site stoichiometry,and discusses the development trend of quantification and stoichiometric analysis methods for phosphorylated proteins.关键词
磷酸化蛋白质/富集方法/质谱/定量方法/化学计量/评述Key words
Phosphorylated protein/Enrichment process/Mass spectrometry/Quantitative approach/Stoichiometry/Review引用本文复制引用
刘媛,翟睿,吴帆,楚占营,赵洋,戴新华,方向,俞晓平..磷酸化蛋白质的定量与化学计量分析方法的研究进展[J].分析化学,2024,52(5):609-623,15.基金项目
国家重点研发计划项目(No.2022YFF0608400)、国家自然科学基金项目(No.21927812)和中国计量科学研究院基本科研业务费项目(Nos.AKY1955,AKY1941,AKY1942)资助 Supported by the National Key R&D Project of China(No.2022YFF0608400),the National Natural Science Foundation of China(No.21927812)and the Fundamental Research Funds of the National Institute of Metrology(Nos.AKY1955,AKY1941,AKY1942). （No.2022YFF0608400）
