分析化学2024，Vol.52Issue(5)：653-663,中插1-中插9,20.DOI:10.19756/j.issn.0253-3820.231353
多波长便携式有机污染物光谱快速检测装置的研制及应用
Development and Application of Portable Multi-wavelength Spectroscopic Rapid Detector for Organic Pollutants
摘要
Abstract
A portable multi-wavelength spectral detector was developed for on-site,rapid and accurate detection of organic pollutants in surface water using ultraviolet light-emitting diodes(UV-LEDs)and the corresponding spectral detection method was built.By measuring the fluorescence spectrum and absorption light intensity and combining with the multi-wavelength spectral correlation calculation method to analyze the target,the developed detector was able to qualitatively and quantitatively detect organic pollutants that responded in the UV-light range of 260-370 nm.The proposed detector was used to detect polycyclic aromatic hydrocarbons(PAHs)and Rhodamine B.The linear ranges of anthracene and Rhodamine B in absorption mode were 10-100 and 8.5-100 mg/L,respectively.In fluorescence mode,the linear ranges for anthracene and Rhodamine B were 0.006-50 and 0.02-0.781 mg/L,correspondingly.The absorption/fluorescence concurrent mode could extend the detection ranges of anthracene and Rhodamine B to 0.006-100 and 0.02-100 mg/L,respectively.The recoveries of PAHs in surface water ranged from 97.4%to 105.1%for anthracene and 84.5%to 91.2%for anthracene,naphthalene,and pyrene in absorption and fluorescence modes,respectively.The method was accurate in both modes.The mixtures containing PAHs were identified by the proposed method under the optimized multi-wavelength spectral correlation calculation.The results demonstrated that the method could achieve a similarity of 75%or more when identifying two different concentrations of solutions containing a single or a mixture of targets within the linear concentration range of the targets.However,the similarity dropped to less than 25%when identifying two solutions of different substances.The proposed method had excellent recognition abilityof targets.The proposed detector and method combined fluorescence and absorption spectroscopy,which could expand the application of spectroscopy in the in situ rapid identification and detection of pollutants such as PAHs in surface water.关键词
荧光光谱/多波长紫外发光二极管/地表水/多环芳烃/快速检测Key words
Fluorescence spectroscopy/Multi-wavelength ultraviolet light-emitting diode/Surface water/Polycyclic aromatic hydrocarbons/Rapid detection引用本文复制引用
陈宇萍,潘佳钏,雷永乾,刘长宇,郏建波,郭鹏然..多波长便携式有机污染物光谱快速检测装置的研制及应用[J].分析化学,2024,52(5):653-663,中插1-中插9,20.基金项目
国家自然科学基金项目(Nos.21974097,22274117,42206043)、广东省重点领域研发计划项目(No.2020B1111350002)、广东省教育厅项目(Nos.2020KSYS004,2020ZDZX2015,2022ZDJS027)和广东省基础与应用基础研究基金项目(No.2023A1515011156)资助. Supported by the National Natural Science Foundation of China(Nos.21974097,22274117,42206043),the R&D Program for Key Areas of Guangdong Province(No.2020B1111350002),the Projects of Guangdong Provincial Department of Education(Nos.2020KSYS004,2020ZDZX2015,2022ZDJS027),and the Project of the Guangdong Provincial Fund for Basic and Applied Fundamental Research(No.2023A1515011156). （Nos.21974097,22274117,42206043）
