分析化学2024，Vol.52Issue(5)：735-742,8.DOI:10.19756/j.issn.0253-3820.231337
定量核磁共振氢谱法测定肺炎球菌荚膜多糖中C多糖杂质的含量
Determination of C-Polysaccharide Content in Streptococcus Pneumoniae Capsular Polysaccharides by Quantitative Proton Magnetic Resonance Spectroscopy
王丽娟 1马庆华 2张秀 1扶晖1
作者信息
- 1. 北京大学化学与分子工程学院,北京大学分析测试中心,北京 100871
- 2. 北京智飞绿竹生物制药有限公司,北京 100176
- 折叠
摘要
Abstract
A quantitative nuclear magnetic resonance(qNMR)method to analyze the content of residual C-polysaccharide(C-Ps)in the Streptococcus pneumoniae capsular polysaccharide was developed.The characteristic peak for C-Ps was confirmed at δH 3.24 by 2D 1H-15N heteronuclear multiple bond correlation(HMBC).By using pneumococcal serotypes 6A,6B and 10A capsular polysaccharids as model samples and dimethyl sulfone as internal standard,the proton qNMR(1H qNMR)absolute quantitation method was established and validated.The linear detection range for C-Ps was 2.5-198 μg/mL(R2>0.999),the limit of quantification was 2.5 μg/mL,and the spiked recoveries were 102%‒109%.The relative standard deviation(RSD)of repeatability and the RSD of 5 days stability of this method were lower than 3%and 1%,respectively.The established 1H qNMR method could be successfully used to determine the absolute C-Ps contents of Streptococcus pneumoniae capsular polysaccharides.This method showed many advantages such as simple operation,good repeatability and robustness,and could be easily adopted for the quality control of Streptococcus pneumoniae capsular polysaccharides during the research and development stages.关键词
定量核磁共振氢谱/肺炎球菌荚膜多糖/C多糖杂质/磷酸胆碱/质量控制Key words
Quantitative proton nuclear magnetic resonance/Streptococcus pneumoniae capsular polysaccharide/C-polysaccharide/Phosphocholine/Quality control引用本文复制引用
王丽娟,马庆华,张秀,扶晖..定量核磁共振氢谱法测定肺炎球菌荚膜多糖中C多糖杂质的含量[J].分析化学,2024,52(5):735-742,8.
