寒旱农业科学2024，Vol.3Issue(5)：476-481,6.DOI:10.3969/j.issn.2097-2172.2024.05.016
胶体金免疫层析法在韭菜农药残留检测中的应用研究
Study on the Application of Colloidal Gold Immunochromatography in the Detection of Pesticide Residues in Chinese Chives
摘要
Abstract
To validate the reliability and applicability of colloidal gold immunochromatographic technology in the detection of pesticide residues in Chinese chives,3 different types of colloidal gold immunochromatographic rapid test products(colloidal gold pesticide rapid test reagents)were used to detect 6 types of pesticide residues,including fludioxonil,imidacloprid,thiamethoxam,carbendazim,cypermethrin,and triazophos,in Chinese chives.These results were compared with those obtained using chromatography-mass spectrometry tandem method,assessing their sensitivity,false positive rate,and false negative rate.The results showed that at 0.5 LOD pesticide addition level,all 3 colloidal gold pesticide rapid test reagents yielded negative results for laboratory controls.However,when the addition levels reached LOD or MLR,the results were positive,demonstrating high sensitivity of the colloidal gold pesticide rapid test reagents,which comply with the national standards for maximum residue limits in food.Laboratory spike tests and actual sample comparison tests showed no significant differences(χ2<3.84)between the colloidal gold rapid detection technology and the chromatography-mass spectrometry method,with both false negative and false positive rates being 0.Therefore,colloidal gold immunochromatographic method can be applied in the rapid detection of pesticide residues in Chinese chives.关键词
韭菜/农药残留/胶体金免疫层析法/快速检测/色谱质谱联用法Key words
Chinese chive/Pesticide residue/Colloidal gold immunochromatography/Rapid test/Chromatography-mass spec-trometry tandem method分类
轻工纺织引用本文复制引用
张继梅,冯文霞,白新明,李亚莉..胶体金免疫层析法在韭菜农药残留检测中的应用研究[J].寒旱农业科学,2024,3(5):476-481,6.基金项目
国家大宗蔬菜产业技术体系兰州综合试验站项目(CARS-23-G-19) （CARS-23-G-19）
甘肃省农产品质量安全监管专项资金(甘农财发[2023]86号). （甘农财发[2023]86号）
