海南医学院学报2024，Vol.30Issue(10)：721-730,10.DOI:10.13210/j.cnki.jhmu.20240402.001
砂仁挥发性成分及其对GES-1细胞保护作用的比较分析
Comparative analysis of the volatile constituents of Amomi fructus and their protective effects on GES-1 cells
摘要
Abstract
Objective:To analyze the volatile components of Amomum villosum(A.villosum)Lour.,Amomum villosum Lour.var.xanthioides T.L.Wu et Senjen and Amomum longiligulare(A.longiligulare)T.L.Wu and their fake species Amomum chinense(A.chinense)Chun,and the gastric mucosal protective activity of the volatile oils of the four(Amomi fructus)species.Methods:Headspace solid-phase microextraction-gaschromatography-mass spectrometry(HS-SPME-GC-MS)was used for the composi-tional analysis of the four(Amomi fructus)species,and the chromatographic results were combined with NIST08 and NIST08s spectral libraries for comparative qualitative characterization,and the relative percentage contents of volatile components were cal-culated by the area normalization method with the principal component analysis and thermogram analysis.The in vitro activity of four(Amomi fructus)species of volatile oils was evaluated by establishing an in vitro human gastric mucosal epithelial cell(GES-1 cell)injury model using the CCK-8 method.Results:From A.villosum Lour.,A.villosum Lour.var.xanthioides T.L.Wu et Senjen,A.longiligulare T.L.Wu and its fake Hainan fake species A.chinense Chun,a total of 114 chemical constituents were detected,and 57,58,58 and 58 volatile constituents were identified,each accounting for 93.23%,88.79%,90.87%,91.40%of the total volatile constituents in terms of the relative percentage of the total volatile constituents content.40.48%,37.77%,37.09%and 33.39%total sesquiterpene hydrocarbons accounted for 52.53%,49.88%,31.22%,35.45%of the relative percentage content of total volatile components,respectively.The volatile oils of A.villosum Lour.、A.villosum Lour.var.xanthioides T.L.Wu et Senjen and A.longiligulare T.L.Wu had proctive activities on ethanol-induced GES-1 cell damage,and the counterfeit A.chi-nense Chun had no significant activity.Conclusion:The differences in volatile components and contents between A.villosum Lour.and A.villosum Lour.var.xanthioides T.L.Wu et Senjen are small,while the differences in volatile components and contents be-tween A.longiligulare T.L.Wu,A.chinense Chu and A.villosum Lour.,A.villosum Lour.var.xanthioides T.L.Wu et Senjen are large.Volatile oils of A.villosum Lour.,A.villosum Lour.var.xanthioides T.L.Wu et Senjen and A.longiligulare T.L.Wu showed protective effects against ethanol-induced damage in human GES-1 cells.关键词
砂仁/挥发性成分/顶空固相微萃取气质联用/GES-1细胞Key words
Amomi fructus/Volatile constituent/HS-SPME-GC-MS/GES-1分类
医药卫生引用本文复制引用
周小妹,张迎,罗明权,练勇,程倩颖,靳德军,孔奕丹,魏娜..砂仁挥发性成分及其对GES-1细胞保护作用的比较分析[J].海南医学院学报,2024,30(10):721-730,10.基金项目
This study was supported by Natural Science Foundation of Hainan Province(822RC705) （822RC705）
The Key Research and Development Program of Hainan Province(ZDYF2022SHFZ127) （ZDYF2022SHFZ127）
Hainan Graduate Student Innovation Research Project(Qhys2021-369) （Qhys2021-369）
Graduate Innovative Research Project of Hainan Medical University(HYYS2021A10) 海南省自然科学基金(822RC705) （HYYS2021A10）
海南省重点研发项目(ZDYF2022SHFZ127) （ZDYF2022SHFZ127）
海南省研究生创新科研课题(Qhys2021-369) （Qhys2021-369）
海南医学院研究生创新科研课题(HYYS2021A10) （HYYS2021A10）
