砂仁挥发性成分及其对GES-1细胞保护作用的比较分析OA北大核心CSTPCD
Comparative analysis of the volatile constituents of Amomi fructus and their protective effects on GES-1 cells
目的:分析阳春砂、绿壳砂、海南砂及其伪品海南假砂的挥发性成分,比较四种砂仁挥发油对胃黏膜保护的活性.方法:采用顶空固相微萃取技术与气相色谱-质谱联用技术(HS-SPME-GC-MS),结合NIST08和NIST08s谱库对比定性色谱结果,分析4种砂仁的挥发性成分;采用面积归一化法,计算各挥发性成分的相对百分比含量,并进行主成分及热图分析;建立人胃黏膜上皮细胞(GES-1细胞)损伤模型,采用CCK-8法,对4种砂仁挥发油进行胃黏膜保护活性评价.结果:从阳春砂、绿壳砂、海南砂及其海南假砂中共检出 114种化学成分,分别鉴定出 57、58、58、58种成分,各占总挥发性成分相对百分比含量的 93.23%、88.79%、90.87%、91.40%,其中总单萜烃类化合物各占总挥发性成分相对百分比含量的 40.48%、37.77%、37.09%、33.39%,总倍半萜烃类化合物各占总挥发性成分相对百分比含量的52.53%、49.88%、31.22%、35.45%;阳春砂、绿壳砂、海南砂挥发油对乙醇致GES-1细胞损伤具有保护作用(P<0.05),海南假砂无明显活性.结论:阳春砂与绿壳砂的挥发性成分及含量差异较小,而海南砂、海南假砂与阳春砂、绿壳砂的挥发性成分及含量差异较大;阳春砂、绿壳砂、海南砂挥发油具有一定的胃黏膜保护活性.
Objective:To analyze the volatile components of Amomum villosum(A.villosum)Lour.,Amomum villosum Lour.var.xanthioides T.L.Wu et Senjen and Amomum longiligulare(A.longiligulare)T.L.Wu and their fake species Amomum chinense(A.chinense)Chun,and the gastric mucosal protective activity of the volatile oils of the four(Amomi fructus)species.Methods:Headspace solid-phase microextraction-gaschromatography-mass spectrometry(HS-SPME-GC-MS)was used for the composi-tional analysis of the four(Amomi fructus)species,and the chromatographic results were combined with NIST08 and NIST08s spectral libraries for comparative qualitative characterization,and the relative percentage contents of volatile components were cal-culated by the area normalization method with the principal component analysis and thermogram analysis.The in vitro activity of four(Amomi fructus)species of volatile oils was evaluated by establishing an in vitro human gastric mucosal epithelial cell(GES-1 cell)injury model using the CCK-8 method.Results:From A.villosum Lour.,A.villosum Lour.var.xanthioides T.L.Wu et Senjen,A.longiligulare T.L.Wu and its fake Hainan fake species A.chinense Chun,a total of 114 chemical constituents were detected,and 57,58,58 and 58 volatile constituents were identified,each accounting for 93.23%,88.79%,90.87%,91.40%of the total volatile constituents in terms of the relative percentage of the total volatile constituents content.40.48%,37.77%,37.09%and 33.39%total sesquiterpene hydrocarbons accounted for 52.53%,49.88%,31.22%,35.45%of the relative percentage content of total volatile components,respectively.The volatile oils of A.villosum Lour.、A.villosum Lour.var.xanthioides T.L.Wu et Senjen and A.longiligulare T.L.Wu had proctive activities on ethanol-induced GES-1 cell damage,and the counterfeit A.chi-nense Chun had no significant activity.Conclusion:The differences in volatile components and contents between A.villosum Lour.and A.villosum Lour.var.xanthioides T.L.Wu et Senjen are small,while the differences in volatile components and contents be-tween A.longiligulare T.L.Wu,A.chinense Chu and A.villosum Lour.,A.villosum Lour.var.xanthioides T.L.Wu et Senjen are large.Volatile oils of A.villosum Lour.,A.villosum Lour.var.xanthioides T.L.Wu et Senjen and A.longiligulare T.L.Wu showed protective effects against ethanol-induced damage in human GES-1 cells.
周小妹;张迎;罗明权;练勇;程倩颖;靳德军;孔奕丹;魏娜
海南医学院海南省热带药用植物研究开发重点实验室,海南 海口 571199||海南医学院药学院,海南 海口 571199
中医学
砂仁挥发性成分顶空固相微萃取气质联用GES-1细胞
Amomi fructusVolatile constituentHS-SPME-GC-MSGES-1
《海南医学院学报》 2024 (010)
721-730 / 10
This study was supported by Natural Science Foundation of Hainan Province(822RC705);The Key Research and Development Program of Hainan Province(ZDYF2022SHFZ127);Hainan Graduate Student Innovation Research Project(Qhys2021-369);Graduate Innovative Research Project of Hainan Medical University(HYYS2021A10) 海南省自然科学基金(822RC705);海南省重点研发项目(ZDYF2022SHFZ127);海南省研究生创新科研课题(Qhys2021-369);海南医学院研究生创新科研课题(HYYS2021A10)
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