海南医学院学报2024，Vol.30Issue(10)：762-768,7.DOI:10.13210/j.cnki.jhmu.20240220.001
Barnesiella intestinihominis菌的安全性评价
Safety evaluation of Barnesiella intestinihominis bacteria
摘要
Abstract
Objective:To evaluate the biosafety of B.intestinihominis in vivo and in vitro,which was an anaerobic bacterium isolated from the feces of healthy adults and whose growth is inhibited in many metabolic disease states,in order to provide a basis for the research and development of microbial ecological agents for metabolic diseases in the future.Methods:The in vivo safety evaluation was carried out by acute toxicity test by gavage of different doses of B.intestinihominis cultures to Kunming rats for 14 consecutive days to observe whether the animals showed any death and toxicity,as well as changes in body weight,biochemical in-dexes,and abundance of intestinal B.intestinihominis bacteria.In vitro evaluation was performed by hemolytic assay,tolerance as-say of gastric acid and intestinal fluid,and sensitivity assay of 9 antibiotics.Results:None of the experimental groups showed any mortality,toxicity due to gavage of the B.intestinihominis,and the differences in their body weights and organ indices were not sta-tistically significant compared to the control group(P>0.05),nor did they show any pathological changes in the organs.Barnesiel-la intestinihominis did not produce a hemolytic ring on Columbia blood agar plates and was gamma-hemolytic.It was not well toler-ated by gastric acid but well tolerated by intestinal fluids,and was sensitive to chloramphenicol,ampicillin,vancomycin and tetra-cycline,but resistant to streptomycin,erythromycin,kanamycin,gentamicin and clindamycin.Conclusion:Oral administration of B.intestinihominis strains has no toxic effect on mice and has a good in vivo safety profile,it is not well tolerated by gastric acid and is sensitive to antibiotics that have a role for gram-negative bacteria,if used as a microcosmic agent it needs to be verified for its beneficial effects on different diseases as well as the need to make a protective agent.关键词
有益菌/急性毒性实验/安全性评价/药敏实验/Barnesiella intestinihominis菌Key words
Beneficial bacteria/Acute toxicity assay/Safety evaluation/Drug sensitivity assay/Barnesiella intestinihominis分类
医药卫生引用本文复制引用
王玲,黄冰,关亚群,热比亚·努力..Barnesiella intestinihominis菌的安全性评价[J].海南医学院学报,2024,30(10):762-768,7.基金项目
This study was supported by National Natural Science Foundation of China(81860743) （81860743）
Basic Research Project of Universities of Autonomous Region(XJEDU2023P064) （XJEDU2023P064）
Tianshan Innovation Team Project(2022D14009) 国家自然科学基金资助项目(81860743) （2022D14009）
自治区高校基本科研项目(XJEDU2023P064) （XJEDU2023P064）
天山创新团队项目(2022D14009) （2022D14009）
