Barnesiella intestinihominis菌的安全性评价OA北大核心CSTPCD

Objective:To evaluate the biosafety of B.intestinihominis in vivo and in vitro,which was an anaerobic bacterium isolated from the feces of healthy adults and whose growth is inhibited in many metabolic disease states,in order to provide a basis for the research and development of microbial ecological agents for metabolic diseases in the future.Methods:The in vivo safety evaluation was carried out by acute toxicity test by gavage of different doses of B.intestinihominis cultures to Kunming rats for 14 consecutive days to observe whether the animals showed any death and toxicity,as well as changes in body weight,biochemical in-dexes,and abundance of intestinal B.intestinihominis bacteria.In vitro evaluation was performed by hemolytic assay,tolerance as-say of gastric acid and intestinal fluid,and sensitivity assay of 9 antibiotics.Results:None of the experimental groups showed any mortality,toxicity due to gavage of the B.intestinihominis,and the differences in their body weights and organ indices were not sta-tistically significant compared to the control group(P>0.05),nor did they show any pathological changes in the organs.Barnesiel-la intestinihominis did not produce a hemolytic ring on Columbia blood agar plates and was gamma-hemolytic.It was not well toler-ated by gastric acid but well tolerated by intestinal fluids,and was sensitive to chloramphenicol,ampicillin,vancomycin and tetra-cycline,but resistant to streptomycin,erythromycin,kanamycin,gentamicin and clindamycin.Conclusion:Oral administration of B.intestinihominis strains has no toxic effect on mice and has a good in vivo safety profile,it is not well tolerated by gastric acid and is sensitive to antibiotics that have a role for gram-negative bacteria,if used as a microcosmic agent it needs to be verified for its beneficial effects on different diseases as well as the need to make a protective agent.