化工学报2024，Vol.75Issue(4)：1382-1393,12.DOI:10.11949/0438-1157.20231395
合成生物学改造酵母驱动丁二酸绿色生物制造
Synthetic biology of yeasts drives green biomanufacturing of succinic acid
摘要
Abstract
Succinic acid is an important C4 platform chemical,and its industrial biomanufacturing route has significant advantages,which is a research hotspot.The microorganisms for succinic acid biosynthesis mainly include bacteria and yeasts.Compared with bacteria,although the product yield of yeasts is lower,the yeasts generally have high acid tolerance,therefore,they can synthesize succinic acid under low pH conditions,thus avoiding adding additional neutralization agent to maintain the neutral pH conditions,therefore,the downstream process for yeast-based succinic acid purification is simpler and costs lower.This article reviews the research progress in the use of yeast microorganisms to synthesize succinic acid in recent years,focusing on the synthetic biology transformation strategies of yeast production strains represented by Saccharomyces cerevisiae,Yarrowia lipolytica,and Issatchenkia orientalis.Finally,the future directions for green biomanufacturing of succinic acid by constructing yeast cell factories are proposed.关键词
酵母/丁二酸/细胞工厂/代谢工程/合成生物学/生物技术Key words
yeast/succinic acid/microbial cell factory/metabolic engineering/synthetic biology/biotechnology分类
生物科学引用本文复制引用
孙涛,孙美莉,陆然,余一梓,王凯峰,纪晓俊..合成生物学改造酵母驱动丁二酸绿色生物制造[J].化工学报,2024,75(4):1382-1393,12.基金项目
国家重点研发计划项目(2021YFC2101100) （2021YFC2101100）
国家自然科学基金优秀青年科学基金项目(21922806) （21922806）
国家自然科学基金面上项目(22178173) （22178173）
