酶的理性设计在作物育种中的应用进展与前景展望OA北大核心CSTPCD

Excellent germplasm resources are important guarantee for food security,and traditional breeding techniques have problems such as long breeding cycles and intensive workloads.With the deep integration of biology and computer technology,the rational design of enzyme used for directional optimization of key crop genes has laid the foundation for greater exploitation of high-quality genetic resources.It further integrates with gene editing and transgenic technologies,and becomes one of the important directions in the exploration of molecular breeding technology.This article elaborated the common methods of rational design of enzyme,such as molecular docking,molecular dynamic simulations,binding free energy evaluation and so on,reviewed the progress on application of rational design of enzyme in improvement of crop quality,stress resistance and biomass,and the future was prospected,to provide reference for the development and utilization of new technologies in crop breeding.