河南农业科学2024，Vol.53Issue(5)：101-111,11.DOI:10.15933/j.cnki.1004-3268.2024.05.011
园林废弃物木质纤维素高效降解真菌的筛选与鉴定
Screening and Identification of Efficient Degradation Fungi for Garden Waste Lignocellulose
摘要
Abstract
In order to screen fungal strains for efficient degradation of lignocellulose in garden waste,PDA medium was used to separate fungi from humus,cow manure,horse manure,dead branches and leaves,compost,straw and other environments and aniline blue medium and filter paper inorganic salt medium were used to qualitatively screen fungal strains with lignocellulosic degrading ability.The activity of lignocellulosic degrading enzymes and their degradation rate on garden waste were quantitatively measured,and excellent fungal strains for efficient degradation of lignocellulose in garden waste were screened.The results showed that a total of 109 strains of fungi were isolated.Among them,there were 32 fungi with lignin degradation ability,accounting for 29.36％of the tested strains;There were 40 fungi with cellulose degradation ability,accounting for 36.70％of the tested strains.Among the above strains,BF31-32,BF32-93,T2-55,T1-16,and T1-24 could efficiently degrade lignocellulose from garden waste,with degradation rates of 50.30％,45.94％,44.49％,44.68％,and 46.81％,respectively.Based on the lignocellulosic degrading enzyme activity,morphological characteristics,and molecular identification results of fungi,strain BF31-32 was identified as Penicillium brevicompactum,strain BF32-93 as Aspergillus fumigatus,strains T1-16 and T1-24 as Fusarium equiseti,and strain T2-55 as Paecilomyces lilacinus.关键词
园林废弃物/木质素/纤维素/酶活性/生物降解真菌Key words
Garden waste/Lignin/Cellulose/Enzyme activity/Biodegradable fungi分类
农业科技引用本文复制引用
黄秋月,何建清,刘海鑫,聂子芳,徐东,潘长漭..园林废弃物木质纤维素高效降解真菌的筛选与鉴定[J].河南农业科学,2024,53(5):101-111,11.基金项目
西藏自治区重点科技项目(XZ202001ZY0019N) （XZ202001ZY0019N）
西藏农牧学院研究生创新计划项目(YJS2023-42) （YJS2023-42）
