西地那非对脑出血所致病理损伤和神经功能缺损的影响OACSTPCD

Objective To investigate the effects of silndenafil on brain damage and neurological function in mice with cerebral hemorrhage.Methods Twenty-four C57Bl/6 mice aged 2 to 3 months were used to construct an intracerebral hemorrhage(ICH)model using stereotaxic frame injection of collagenase into the brain.The ICH mice were randomly divided into the vehicle group and the sildenafil group,and were respectively given normal saline and equal volume of 0.5 mg/kg sildenafil in normal saline by oral dosage in 7 days following ICH.Then,mNSS scores were performed on days 3,7 and 14.Brain magnetic resonance imaging was performed on day 7 to detect cerebral hematoma volume,and laser speckle flowmeter was used to analyze cerebral blood flow.The mice were sacrificed on the 14th day after modeling,and the hemoglobin content in the brain was detected using a hemoglobin detection kit.Results The neurological deficit scores of the mice in the sildenafil treatment group(6.17±1.19,2.75±1.21)were significantly smaller than those in the vehicle group(8.50±1.12,4.83±0.93)on days 7 and 14(F=6.49,P<0.001).The intracerebral hematoma volume and the corrected brain swelling in the sildenafil group(2.94±1.05,5.37±1.26)were significantly smaller than that of the vehicle-treated group(6.03±1.23,8.01±1.75)on day 7(t=4.66,2.99,respectively,P<0.05).Relative cerebral blood flow in the sildenafil group(82.50±5.17)was significantly higher than that of the vehicle group(73.50±7.56,P<0.05).The hemoglobin content in the brain of mice in the sildenafil treatment group(36.98±12.31)was significantly reduced compared with the vehicle group(66.00±17.21,P<0.05).In addition,pathology investigation revealed that the immunoactivity of the endothelial cell marker CD31 was significantly higher in the SDNF group(34.72±8.22)than that of the vehicle treated group(23.00±8.60)on the 14th day(P<0.05).Conclusion Sildenafil can improve the symptoms of neurological deficit in mice after cerebral hemorrhage,improve cerebral blood flow,promote hematoma absorption,and reduce the content of hemoglobin in the brain.Sildenafil has potential clinical application value in the treatment of cerebral hemorrhage.