杉木GRF和GIF基因家族鉴定、表达与互作分析OA北大核心CSTPCD

Growth regulators(GRF)and GRF-interacting factors(GIF)play crucial roles in plant growth,development,and response to stress.In order to study the functions of GRF and GIF genes in the growth,development and stress response of Cunninghamia lanceolata,we identified five ClGRFs genes and two ClGIFs genes based on the full-length transcriptome data of C.lanceolata.Our analysis revealed that the deduced amino acid sequence of ClGRFs ranged from 386 aa(ClGRF4)to 723 aa(ClGRF3),with isoelectric points between 7.07(ClGRF4)and 9.26(ClGRF5).The two ClGIF proteins consisted of 242 and 258 aa,respectively,with isoelectric points around 6.Conserved domain analysis showed that all ClGRFs proteins contained QLQ and WRC domains,and all ClGIFs proteins contained SSXT domains.Phylogenetic analysis placed ClGRFs in branch III and branch VI,respectively.And,all the ClGRF genes were regulated by miR396.Further analysis indicated that the expressions of ClGRF genes showed tissue specificity,while the ClGIF genes were constitutively expressed in all detected tissues.Moreover,both ClGRF and ClGIF genes can respond to GA3 and SA treatment.Yeast two-hybrid experiments indicated the physical interactions between all ClGRFs proteins and the two ClGIFs proteins.These results provide an important basis for further studies on the functions of GRF and GIF genes in C.lanceolata.