棉花独脚金内酯D27基因表达与功能分析OA北大核心CSTPCD

To investigate the biological functions of D27 gene in cotton fiber and plant development,18 members of D27 gene in cotton genus were identified through bioinformatics methods,and the physicochemical properties,structures,and evolutionary relationships were analyzed.The expression specificity of six GbD27 genes in island cotton fibers was investigated using quantitative real-time PCR(qRT-PCR)technology.Six GhD27 genes in upland cotton were obtained through homologous recombination.A VIGS vector of the GhD27 gene were constructed and transformed into cotton.The silencing efficiency and expression patterns of GhD27 under treatment with monophyllone were detected using qRT-PCR technology,and the endogenous monophyllone content in silenced plants were determined suing ELISA technology.The results showed that the open reading frame(ORF)length of cotton D27 gene family was 726-816 bp,and the encoded D27 protein contained 241-271 amino acids.D27 protein is mainly composed of alpha helix and random curl.The evolutionary analysis results show that the D27 gene is highly homologous.The GbD27 gene in island cotton material XH 21 is expressed in cotton fibers from 0 to 35 days old,showing possibly important regulatory role in cotton fiber development.The GhD27 gene of upland cotton material XND cotton 4 was effectively silenced in both leaves and roots,indicating that the GhD27 gene silenced plants have been obtained.In the silenced plants of the GhD27 gene in upland cotton,the GhD27 gene can respond to SLs treatment,and there is a certain correlation between endogenous SLs content and the expression level of GhD27 gene family.This study lays a foundation for further understanding the molecular biological functions of cotton D27 gene.