奶牛妊娠中后期胎盘mRNA差异表达基因分析OA北大核心CSTPCD

Reproduction of dairy cows is a bottleneck that restricts the rapid development of China's dairy industry.In order to study the differential gene expression in the placenta of cows in mid and late gestation,and to explore the regulatory role of mRNA in the process of cow pregnancy,the placenta of cows in mid and late gestation,and the uterus of non-pregnant control group was used as the study objects,and transcriptome analysis was performed using RNA-seq sequencing technology.The results revealed that 4 333 and 4 354 genes showed significantly differential expressions in the mid and late-gestation placentas,respectively,compared with the non-pregnant control group,with 2 328 and 2 519 genes up-regulated and 2 005 and 1 835 down-regulated genes,respectively.778 differentially expressed genes existed only in the mid-pregnancy,and 757 differentially expressed genes existed only in the late-pregnancy.GO analysis indicated that the differentially expressed genes in mid and late gestation were mainly enriched in the processes of localization,bioadhesion,cell junction,and reproduction.KEGG analysis showed that the differentially expressed genes in mid and late gestation were enriched in the pathways of ECM-receptor interactions,cell adhesion molecules,cell cycle,adhesion patches,PI3K-Akt signaling pathway,pathways of cancer,and digestion and absorption of proteins.Between the middle and late stages of pregnancy,there were obvious differences in the expression of genes,including PLET1,CSH1,SULT1E1,PRP1,LPAR2,CSF1R,PDCD1,which were up-regulated,and it was hypothesized that they might be involved in the regulation of pregnancy in cows,and influence the occurrence of pregnancy to a certain extent.The results of this study provide a theoretical basis for the in-depth excavation of the molecular regulatory mechanisms in the middle and late stages of pregnancy in dairy cows.