禽腺病毒血清4型Hexon蛋白的转录组学功能分析OA北大核心CSTPCD

Fowl adenovirus serotype 4(FAdV-4)is an important pathogen causing avian hepatitis and pericardial effusion syndrome,which seriously endangers the development of poultry industry in our country.Current evidence has suggested that the FAdV-4 Hexon protein was one of the important components determining viral virulence and pathogenicity.In order to further explore the critical role of Hexon protein in FAdV-4 infection,this study selected Leghorn male hepatocellular(LMH)cells as research model,and transcriptomic sequencing technology was used to screen the differentially expressed genes induced by Hexon overexpression.The results showed that compared with the control group,445 differentially expressed genes were up-regulated and 36 differentially expressed genes were down-regulated.Among them,BAG3,JUN,IL8L1,CCL4 and THBS1 genes have previously been described to be associated with FAdV-4 infection,while the functions of HSPB9,HSP90AA1,HSPA8,HSPA2,DCN,ELOVL3,and SBK2 genes in FadV-4 infection have not been reported.GO enrichment analysis showed that down-regulated differential genes were mainly related to early endosome and proteasomal accessory complexes,while up-regulated differential genes were mainly related to plasma membrane and extracellular surface regions and supramolecular fibers.KEGG pathway enrichment analysis showed that differentially expressed genes were mainly enriched in MAPK,Wnt,VEGF and other signaling pathways.In addition,several key proteins such as HSP90AA1,HSPA8,JUN,and DCN were found to be positioned at the center of the whole network by protein-protein interaction(PPI)network analysis and have been described to be closely involved in viral replication.In summary,our study revealed a series of differentially expressed genes induced by Hexon overexpression through RNA sequencing,which may provide new targets and theoretical basis for controlling FAdV-4 infection.