湖南农业大学学报（自然科学版）2024，Vol.50Issue(2)：42-49,8.DOI:10.13331/j.cnki.jhau.2024.02.007
三桠苦叶绿体基因组结构和序列特征分析
Analysis on structure and sequence feature of chloroplast genome from Melicope pteleifolia
摘要
Abstract
To illustrate the structure and sequence characteristics of chloroplast genome from Melicope pteleifolia,the leaf of M.pteleifolia was used for DNA isolation and library construction.The high-throughput sequencing was fulfilled by Novaseq platform,and then acquired sequence was assembled,annotated and analyzed using bioinformatics means.The results revealed that the chloroplast genome of M.pteleifolia was a circular quadripartite molecule with a size of 158 992 bp and encoded 134 genes.Altogether 86 simple sequence repeat was found in the plastome and the A/T mononucleotide was predominate type.Total 26 907 codon was screened out and those ended with A/T were frequently used.Sequence comparison demonstrated more divergence and occurred in non-coding region between M.pteleifolia and other Rutaceae species.Phylogenetic tree indicated various chloroplast genome sequences of M.pteleifolia shared close relationship and exhibited diversity in gene composition and base sequence.关键词
三桠苦/叶绿体基因组/结构特征/序列比对Key words
Melicope pteleifolia/chloroplast genome/structure feature/sequence alignment分类
医药卫生引用本文复制引用
吴民华,陈依虹,谭靖怡,邹山扬,叶晓霞,黄琼林..三桠苦叶绿体基因组结构和序列特征分析[J].湖南农业大学学报（自然科学版）,2024,50(2):42-49,8.基金项目
广东省基础与应用基础研究基金项目(2018A030310116) （2018A030310116）
广东省大学生创新创业训练计划项目(S202310571113) （S202310571113）
湛江市科技计划项目(2019A01008) （2019A01008）
广东医科大学大学生创新创业训练计划项目(GDMU2022113) （GDMU2022113）
