湖南农业大学学报（自然科学版）2024，Vol.50Issue(2)：50-56,7.DOI:10.13331/j.cnki.jhau.2024.02.008
青钱柳叶片甜味性状差异的转录组比较分析
Comparative transcriptome analysis of sweetness traits difference of Cyclocarya paliurus
摘要
Abstract
In this study,leaves of sweet and non-sweet taste Cyclocarya paliurus leaves planted in Wugang,Hunan Province were selected for comparative study to illustrate the mechanism of sweet taste formation.The cDNA libraries were constructed and sequenced.The transcriptome date was analyzed with there terpenoid metabolome and the metabolism pathway.The molecular differences in the formation of sweetness traits of Cyclocarya paliurus were explored.Some 13 key enzyme genes involved in triterpene biosynthesis to have significantly different expression patterns between the two materials by GO and KEGG analysis.Combined with terpene metabolism detection,the accumulation of four kinds of sweet triterpenes in sweet Cyclocarya paliurus leaves was significantly higher than that in non-sweet leaves,indicating that the differential expression of these genes maybe causing different accumulation of sweet triterpenoids in leaves,it's key to the formation of sweetness traits in Cyclocarya paliurus.关键词
青钱柳/转录组/三萜类化合物/甜味性状Key words
Cyclocarya paliurus/transcriptome/triterpene/sweetness traits分类
农业科技引用本文复制引用
廖李玲,张学文,赵燕,陆明亮,刘芬,杨华..青钱柳叶片甜味性状差异的转录组比较分析[J].湖南农业大学学报（自然科学版）,2024,50(2):50-56,7.基金项目
农业农村部项目(XCZX-2023141) （XCZX-2023141）
湖南硒海生物科技有限公司项目(2020kjc-js170) （2020kjc-js170）
