湖南农业大学学报（自然科学版）2024，Vol.50Issue(2)：71-77,7.DOI:10.13331/j.cnki.jhau.2024.02.011
黔北黑猪骨骼肌中肌纤维类型转化相关circRNAs的筛选
Screening of circRNAs related to muscle fiber type conversion in skeletal muscle of Qianbei black pigs
摘要
Abstract
Nine 6-month-old healthy Qianbei black pigs were randomly divided into 3 groups.The longissimus dorsi muscle(LDM)and psoas major muscle(PMM)were collected.The expression profiles of circRNAs were constructed by RNA-seq,and the differentially expressed circRNAs in LDM vs.PMM were screened.A total of 63 differentially expressed circRNAs were identified in the two muscles of Qianbei black pigs,of which 31 were up-regulated and 32 were down-regulated in LDM.GO enrichment and KEGG analysis were performed on the source genes of differentially expressed circRNAs.The results showed that the source genes of differentially expressed circRNAs were mainly concentrated in GO items such as the regulation of phosphatase activity,sarcomere activity,and positive regulation of myosin-light-chain-phosphatase activity.In the enriched KEGG pathway,the pathways involving muscle fiber type conversion include the regulation of actin cytoskeleton,ECM-receptor interaction,PI3K-Akt signaling pathway.Finally,target miRNAs of differentially expressed circRNAs were predicted,and corresponding target mRNAs of miRNAs were screened to construct the circRNA-miRNA-mRNA regulatory network.The results showed that circ_0003749-miR-27a-MSTN,circ_0003749-miR-27a-PPARγ,circ_0012316-miR-23a-Myh1/2/4,circ_0007093-miR-214-3p-MEF2C,circ_0010118-miR-132-FoxO1 and circ_0010118-miR-374a-5p-FoxO1 may regulate muscle fiber type conversion through ceRNA network.关键词
黔北黑猪/骨骼肌/肌纤维类型转化/circRNA-miRNA-mRNA网络Key words
Qianbei black pigs/skeletal muscle/muscle fiber type conversion/circRNA-miRNA-mRNA network分类
农业科技引用本文复制引用
赵园园,孟金柱,宋兴超,安清明,吴震洋..黔北黑猪骨骼肌中肌纤维类型转化相关circRNAs的筛选[J].湖南农业大学学报（自然科学版）,2024,50(2):71-77,7.基金项目
贵州省教育厅青年科技人才成长项目(黔教合KY字[2020]166) （黔教合KY字[2020]166）
贵州省科学技术厅高层次创新型人才培养项目(千层次)(2022-(2020)-044) （千层次）
贵州省科学技术厅重点实验室项目(黔科合平台人才[2020]2003号) （黔科合平台人才[2020]2003号）
铜仁市科技局科技支撑计划项目(铜市科研[2020]128号) （铜市科研[2020]128号）
