湖南农业大学学报（自然科学版）2024，Vol.50Issue(2)：78-85,8.DOI:10.13331/j.cnki.jhau.2024.02.012
基于GBS测序的湘东黑山羊的亲缘关系及近交系数
Genetic relationship and inbreeding coefficient among Xiangdong black goats based on GBS sequencing
摘要
Abstract
In this study,60 Xiangdong black goats,including 9 rams and 51 ewes,were sequenced through GBS sequencing,for SNPs and ROH on the chromosomes of Xiangdong black goats.Genetic relationship analysis based on G matrix and NJ cluster analysis were carried out using SNP data to construct pedigree of population structure and inbreeding coefficient was obtained based on ROH data.These results showed that average sequencing depth of 60 Xiangdong black goats was 9.15X,with a mapping rate of 99.59%compared with reference genomes.In the process of SNPs detection,the data of 3 ewes failed to meet the genotype quality control standards thus were excluded to obtain the 153046 SNPs and 1937 ROH fragments on 29 autosomes from 57 Xiangdong black goats,based on which pedigree including 8 families was constructed in which 9 rams and 6 ewes were divided into 7 families and other 42 ewes were classified separately.Based on ROH,the average inbreeding coefficient of individual in the population of Xiangdong black goat was 0.047,indicating that the rams have good potential for breeding.关键词
湘东黑山羊/基因分型测序/单核苷酸多态性/连续纯合子片段/近交系数/亲缘关系Key words
Xiangdong black goat/genotyping by sequencing/single nucleotide polymorphism(SNP)/runs of homozygosity(ROH)/inbreeding coefficient/genetic relationship分类
农业科技引用本文复制引用
刘海林,江为民,段洪峰,李昊帮,罗璋,李安定,龚龑..基于GBS测序的湘东黑山羊的亲缘关系及近交系数[J].湖南农业大学学报（自然科学版）,2024,50(2):78-85,8.基金项目
湖南省科学技术厅重点研发计划项目(2020NK2049) （2020NK2049）
