化学工程2024，Vol.52Issue(5)：6-12,7.DOI:10.3969/j.issn.1005-9954.2024.05.002
石油降解菌株的生物炭固定化及性能研究
Biochar immobilization and performance of petroleum degrading strains
摘要
Abstract
Immobilized bacteria agent is an ideal material for degradation of organic pollutants.Using shell-based biochar as carrier and efficient petroleum degrading bacteria as target microorganism,immobilized petroleum degrading bacteria was prepared by adsorption method.The effects of inoculation amount,carbon dosage,time,temperature and shaking speed on fixation rate were investigated,and the optimum preparation conditions of inoculant were determined by orthogonal experiment and response surface analysis.The oil removal properties of biochar,free bacteria and immobilized bacteria were investigated with dosage and oil mass fraction as variables.The results show that under the conditions of 0.3 g carbon dosage,2.26 mL inoculation amount,fixation for 8.462 h,rotation speed of 38.331 r/min,30 ℃,the fixation rate is the best,reaching 87.261％.The main and secondary factors affecting the fixation rate are:inoculation amount＞shaking speed＞immobilization time＞carbon dosage.The overall degradation capacity of the immobilized bacteriological agent for petroleum is 74％-84％,which is better than that of free bacteria and biochar,indicating that the immobilized bacteriological agent has a good application prospect for the bio-remediation of petroleum contaminated soil.关键词
固定化菌剂/石油降解菌/响应面分析法/石油降解率Key words
immobilized bacterial agent/petroleum degrading bacteria/response surface methodology/oil degradation rate分类
资源环境引用本文复制引用
林婷,李海红,温家峰..石油降解菌株的生物炭固定化及性能研究[J].化学工程,2024,52(5):6-12,7.基金项目
陕西省科技厅项目一般项目——农业领域(2024NC-YBXM-240) （2024NC-YBXM-240）
西安市科学技术局农业技术攻关项目(23NYGG0045) （23NYGG0045）
陕西省教育厅服务地方项目(23JC034) （23JC034）
