华西口腔医学杂志2024，Vol.42Issue(3)：286-295,10.DOI:10.7518/hxkq.2024.2023336
骨膜蛋白在小鼠牙周炎进程中的时空表达规律研究
Temporal and spatial expression analysis of periostin in mice periodontitis model
摘要
Abstract
Objective This study aimed to investigate the temporal and spatial changes in the expression of perios-tin during periodontal inflammation in mice.Methods A periodontitis model was constructed using silk thread ligation.Mice were randomly divided into five groups in-cluding control group,4-day ligation group,7-day liga-tion group,14-day ligation group,and self-healing group(thread removal for 14 days after 14-day ligation).Mi-cro-CT and histological staining were performed to char-acterize the dynamic changes in the mouse periodontal tissue in each group.RNAscope and immunohistochemical staining were used to analyze the pattern of changes in peri-ostin at various stages of periodontitis.The cell experiment was divided into three groups:control group,lipopolysac-charide(LPS)stimulation group(treated with LPS for 12 h),and LPS stimulation removal group(treated with LPS for 3 h followed by incubation with medium for 9 h).Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of periostin,transforming growth factor-β1(TGF-β1),and matrix metalloproteinase 2(MMP2).Results Significant alveolar bone resorption was observed 7 days after ligation.With increasing duration of ligation,the damage to the mouse periodontal tissue was aggravated,which manifested as increased osteoclasts,widen-ing of the periodontal membrane space,and decreased alveolar bone height.Some degree of periodontal tissue repair was observed in the self-healing group.Periostin expression decreased at 4 and 7 days compared with the control group and increased at 14 days compared with 4 and 7 days.A significant recovery was found in the self-healing group.The qRT-PCR results showed that the expression of periostin and TGF-β1 in the LPS stimulation group decreased compared with that in the control group but significantly recovered in the LPS removal group.Conclusion Periostin expression in the PDL of mice showed a downward and upward trend with inflammation progression.The significant recovery of periostin expression after removing inflammatory stimuli may be related to TGF-β1,which is crucial to maintain the in-tegrity of the PDL.关键词
牙周炎/骨膜蛋白/牙周再生/牙槽骨/动物模型Key words
periodontitis/periostin/periodontal regeneration/alveolar bone/animal model分类
医药卫生引用本文复制引用
李钺,许春梅,谢旭东,施培磊,王骏,丁一..骨膜蛋白在小鼠牙周炎进程中的时空表达规律研究[J].华西口腔医学杂志,2024,42(3):286-295,10.基金项目
The National Natural Science Fundation of China(82071127,82270990) （82071127,82270990）
Sichuan Key Research and Development Program(2023YFS0032) （2023YFS0032）
Fundamental Research Fundation for the Central Universities,Research and Development Program of West China Hospital of Stomatology,Sichuan University(RD-02-202403) （RD-02-202403）
Natural Science Foundation of Sichuan Province(2022NSFSC0795)国家自然科学基金(82071127,82270990) （2022NSFSC0795）
四川省重点研发计划(2023YFS0032) （2023YFS0032）
中央高校基本科研业务费专项基金四川大学华西口腔医院探索与研发项目(RD-02-202403) （RD-02-202403）
四川省自然科学基金(2022NSFSC0795) （2022NSFSC0795）
