|国家科技期刊平台
首页|期刊导航|华西口腔医学杂志|GRP78-c-Src信号通路介导周期性牵张力作用下牙周膜成纤维细胞成骨分化的机制研究

GRP78-c-Src信号通路介导周期性牵张力作用下牙周膜成纤维细胞成骨分化的机制研究OA北大核心CSTPCDMEDLINE

Role of the GRP78-c-Src signaling pathway on osteoblast differentiation of periodontal ligament fibroblasts in-duced by cyclic mechanical stretch

中文摘要英文摘要

目的 探讨在周期性牵张力作用下葡萄糖调节蛋白78(GRP78)对牙周膜成纤维细胞成骨分化的影响,并阐述其机制.方法 应用FlexCell 5000细胞应力装置模拟牙齿正畸受力环境;应用流式细胞术细胞分选技术获得牙周膜成纤维细胞GRP78高表达细胞和GRP78低表达细胞;采用基因转染技术敲减GRP78、c-Src的表达以及过表达c-Src;蛋白质印迹实验检测成骨转录因子Runt相关基因2(RUNX2)、锌指结构转录因子(Osterix)以及成骨标志蛋白骨钙蛋白(OCN)、骨桥蛋白(OPN)的表达;免疫共沉淀实验检测GRP78与c-Src激酶的相互作用;茜素红染色实验检测细胞矿化结节的形成.结果 GRP78在牙周膜成纤维细胞呈异质性表达,流式分选实验获得GRP78高表达和GRP78低表达细胞.周期性牵张力处理后,与GRP78低表达细胞相比,GRP78高表达细胞成骨分化能力及c-Src激酶磷酸化水平均升高,差异具有统计学意义(P<0.05);GRP78与c-Src激酶存在相互作用;与对照组相比,过表达c-Src组细胞成骨分化能力升高(P<0.05),sic-Src组细胞成骨分化能力降低(P<0.05).结论 GRP78通过与c-Src激酶相互作用并上调其表达,进而促进周期性牵张力诱导的牙周膜成纤维细胞成骨分化.

Objective This study aims to investigate the influence of glucose regulated protein(GRP)78 on osteo-blast differentiation in periodontal ligament fibroblasts(PDLFs)under cyclic mechanical stretch and determine the under-lying mechanism.Methods FlexCell 5000 cell mechani-cal device was applied to simulate the stress environment of orthodontic teeth.GRP78High and GRP78Low sub-population were obtained by flow sorting.Gene transfec-tion was performed to knockdown GRP78 and c-Src ex-pression and overexpress c-Src.Western blot analysis was used to detect the protein expression of Runt-related gene 2(RUNX2),Osterix,osteocalcin(OCN),and osteopontin(OPN).Immunoprecipitation assay was used to determine the interaction of GRP78 with c-Src.The formation of cellular mineralized nodules was determined by alizarin red staining.Results GRP78 was heterogeneously expressed in PDLFs,and GRP78High and GRP78Low subpopulations were obtained by flow sorting.The osteogenic differentiation ability and phosphorylation level of c-Src kinase in the GRP78High subpopulation were significantly increased com-pared with those in GRP78Low subpopulation after cyclic mechanical stretch(P<0.05).GRP78 interacted with c-Src in PDLFs.The overexpression c-Src group showed significantly increased osteogenic differentiation ability than the vector group(P<0.05),and the sic-Src group showed significantly decreased osteogenic differentiation ability(P<0.05)after cy-clic mechanical stretch.Conclusion GRP78 upregulates c-Src expression by interacting with c-Src kinase and pro-motes osteogenic differentiation under cyclic mechanical stretch in PDLFs.

胡静;崔智华;黄克强;苏荣健;赵颂

锦州医科大学生命科学研究院公共实验平台,锦州 121001中国医科大学口腔医学院附属口腔医院种植中心,沈阳 110002锦州医科大学附属口腔医院正畸科,锦州 121001锦州医科大学基础医学院,锦州 121001

口腔医学

周期性牵张力成骨分化牙周膜成纤维细胞葡萄糖调节蛋白78

cyclic mechanical stretchosteogenic differentiationperiodontal ligament fibroblastsglucose regu-lated protein 78

《华西口腔医学杂志》 2024 (003)

304-312 / 9

Basic Research Project of Liaoning Provincial Education Department(JYTJCZR2020073);General Pro-ject of Liaoning Provincial Education Department(LJKZ0803)辽宁省教育厅基础研究项目(JYTJCZR2020073);辽宁省教育厅面上项目(LJKZ0803)

10.7518/hxkq.2024.2023354

评论