华西口腔医学杂志2024，Vol.42Issue(3)：304-312,9.DOI:10.7518/hxkq.2024.2023354
GRP78-c-Src信号通路介导周期性牵张力作用下牙周膜成纤维细胞成骨分化的机制研究
Role of the GRP78-c-Src signaling pathway on osteoblast differentiation of periodontal ligament fibroblasts in-duced by cyclic mechanical stretch
摘要
Abstract
Objective This study aims to investigate the influence of glucose regulated protein(GRP)78 on osteo-blast differentiation in periodontal ligament fibroblasts(PDLFs)under cyclic mechanical stretch and determine the under-lying mechanism.Methods FlexCell 5000 cell mechani-cal device was applied to simulate the stress environment of orthodontic teeth.GRP78High and GRP78Low sub-population were obtained by flow sorting.Gene transfec-tion was performed to knockdown GRP78 and c-Src ex-pression and overexpress c-Src.Western blot analysis was used to detect the protein expression of Runt-related gene 2(RUNX2),Osterix,osteocalcin(OCN),and osteopontin(OPN).Immunoprecipitation assay was used to determine the interaction of GRP78 with c-Src.The formation of cellular mineralized nodules was determined by alizarin red staining.Results GRP78 was heterogeneously expressed in PDLFs,and GRP78High and GRP78Low subpopulations were obtained by flow sorting.The osteogenic differentiation ability and phosphorylation level of c-Src kinase in the GRP78High subpopulation were significantly increased com-pared with those in GRP78Low subpopulation after cyclic mechanical stretch(P<0.05).GRP78 interacted with c-Src in PDLFs.The overexpression c-Src group showed significantly increased osteogenic differentiation ability than the vector group(P<0.05),and the sic-Src group showed significantly decreased osteogenic differentiation ability(P<0.05)after cy-clic mechanical stretch.Conclusion GRP78 upregulates c-Src expression by interacting with c-Src kinase and pro-motes osteogenic differentiation under cyclic mechanical stretch in PDLFs.关键词
周期性牵张力/成骨分化/牙周膜成纤维细胞/葡萄糖调节蛋白78Key words
cyclic mechanical stretch/osteogenic differentiation/periodontal ligament fibroblasts/glucose regu-lated protein 78分类
医药卫生引用本文复制引用
胡静,崔智华,黄克强,苏荣健,赵颂..GRP78-c-Src信号通路介导周期性牵张力作用下牙周膜成纤维细胞成骨分化的机制研究[J].华西口腔医学杂志,2024,42(3):304-312,9.基金项目
Basic Research Project of Liaoning Provincial Education Department(JYTJCZR2020073) （JYTJCZR2020073）
General Pro-ject of Liaoning Provincial Education Department(LJKZ0803)辽宁省教育厅基础研究项目(JYTJCZR2020073) （LJKZ0803）
辽宁省教育厅面上项目(LJKZ0803) （LJKZ0803）
