吉林农业大学学报2024，Vol.46Issue(2)：175-186,12.DOI:10.13327/j.jjlau.2021.20297
基因敲除技术在微生物代谢途径改造中的研究进展
Research Progress of Gene Knockout Technology in Modification of Microbial Metabolic Pathway
摘要
Abstract
Microorganisms are excellent hosts for the production of a variety of high value-added natural products.Gene knockout technology can effectively block or weaken the bypass metabolic pathway of microorganisms,so that the metabolic flux can concentrate on target products.The appli-cation of gene knockout technology in microorganisms has promoted the research of microbial meta-bolic engineering and opened up a new way for the development of microbial industrial fermentation and synthetic biology.This paper has mainly reviewed several common microbial gene knockout strategies and summarized the application of gene knockout technology in microbial metabolic path-way modification in recent years.The application prospect of gene knockout technology in the trans-formation of microbial metabolic pathway has been analyzed in order to provide some theoretical ref-erence for the development of gene knockout technology and the research of microbial metabolic en-gineering.关键词
基因敲除技术/代谢工程/合成生物学/底盘微生物Key words
gene knockout technology/metabolic engineering/synthetic biology/chassis microor-ganism分类
农业科技引用本文复制引用
张斯童,石佳,王刚,田春杰,陈光..基因敲除技术在微生物代谢途径改造中的研究进展[J].吉林农业大学学报,2024,46(2):175-186,12.基金项目
中国科学院战略先导科技项目(XDA28020400),吉林省科技发展计划重点项目(20210203117),吉林省自然科学基金项目(20220101334JC) （XDA28020400）
