吉林中医药2024，Vol.44Issue(5)：564-570,7.DOI:10.13463/j.cnki.jlzyy.2024.05.017
无糖型强力枇杷露对雾霾环境致咳豚鼠的改善作用研究
Research on the ameliorating effect of sugar-free Qiangli Loquat Dew on a guinea pig model of coughs induced by haze
摘要
Abstract
Objective To observe the cough-relieving and phlegm-dispelling effect of sugar-free Qiangli Loquat Dew and explore its mechanisms.Methods The guinea pig citric acid induced cough method and guinea pig haze smog induced chronic cough method were respectively used to observe their general state and record the number of coughs in them.HE staining was used to observe the pathological changes in trachea and lung tissues.Under microscope,bronchoalveolar lavage fluid(BALF)cells were classified and counted.The levels of IL-8,TNF-α,SP,Muc5AC,SOD,CAT,MDA,GSH-Px in their lung tissues were detected by ELISA,and its cough-relieving effects and mechanisms were observed.Its phlegm-dispelling effects was studied by the experiment of excretion amount of phenol red in mouse trachea.Results Compared with the model group,the number of coughs in the Suhuang group,the Feilike group,and the low-,medium-,and high-dose groups of Qiangli Loquat Dew were significantly reduced(P<0.05),the antitussive rate was significantly increased(P<0.05),the concentration of phenol red was significantly increased(P<0.05).Compared with the blank group,the total cell concentration in BALF in the model group was significantly increased,the proportion of eosinophil number and lymphocyte number was increased(P<0.05),the contents of IL8,TNF-α,SP,Muc5AC and MDA in lung tissues were significantly increased(P<0.05),while the contents of SOD,CAT and GSH-Px were significantly decreased(P<0.05).Compared with the model group,the total cell concentrations in BALF in the Suhuang group,the Feilike group,and the low-,medium-,and high-dose groups of Qiangli Loquat Dew were decreased to varying degrees,and the proportion of eosinophil number and lymphocyte number was decreased(P<0.05),and the contents of IL-8,TNF-α,SP,Muc5AC,and MDA in lung tissues were all decreased(P<0.05),while the contents of SOD,CAT,GSH-Px were increased significantly(P<0.05).Conclusion Sugar-free Qiangli Loquat Dew can improve the pathological damage of a guinea pig model with chronic coughs induced by haze,reduce airway inflammation,inhibit oxidative stress,enhance the secretion function of the respiratory tract and exert cough-relieving and phlegm-dispelling effects.关键词
无糖型强力枇杷露/雾霾/药效学/炎症因子/氧化应激Key words
sugar-free Qiangli Loquat Dew/haze/pharmacodynamics/inflammatory factors/oxidative stress分类
医药卫生引用本文复制引用
田宇诗,穆滨,艾春林,胡晓阳,李冀..无糖型强力枇杷露对雾霾环境致咳豚鼠的改善作用研究[J].吉林中医药,2024,44(5):564-570,7.基金项目
黑龙江省应用技术研究与开发计划项目(GY2017YD0165) （GY2017YD0165）
中医药传承与创新"百千万"人才工程(岐黄工程)岐黄学者项目(国中医药人教函[2018]284号) （岐黄工程）
