吉林中医药2024，Vol.44Issue(5)：590-594,5.DOI:10.13463/j.cnki.jlzyy.2024.05.022
高效液相色谱法测定益肾排石颗粒中黄芩苷含量
Determination of baicalin in Yishen Paishi granules by HPLC
摘要
Abstract
Objective To establish an HPLC method for the determination of baicalin in Yishen Paishi granules,and to provide a scientific and reliable basis for the quality control of the medicine.Methods High performance liquid chromatography(HPLC)was performed on Shimadzu ODS-2C18 column(250 mm×4.6 mm,5 μm),with its mobile phase as a gradient elution of 0.2%phosphoric acid water(A)-methanol(B),with its flow rate at 1.0 mL/min,its column temperature at 30℃,its detection wavelength at 280 nm,and its sample size all at 10 μL.Results The linear relationship between the peak area and the concentration of baicalin was good,r=1.000 0.Its precision and stability met the requirements.Its average recovery was 103.29%and RSD was 2.38%.The average content of baicalin in the three batches of samples was 6.12 mg/g,and the RSD value was 1.71%.Conclusion The method has a good repeatability and a high accuracy,and can be used for the determination of baicalin in compound Yishen Paishi granules.关键词
益肾排石颗粒/黄芩苷/高效液相色谱法/含量测定Key words
Yishen Paishi granules/baicalin/high performance liquid chromatography(HPLC)/content determination分类
医药卫生引用本文复制引用
马永璠,薄天儒,于亚杰,刘艳华,初洪波..高效液相色谱法测定益肾排石颗粒中黄芩苷含量[J].吉林中医药,2024,44(5):590-594,5.基金项目
吉林省科技发展计划项目(20200404068YY) （20200404068YY）
吉林省自然科学基金项目(YDZJ202201ZYTS237) （YDZJ202201ZYTS237）
