常州大学学报（自然科学版）2024，Vol.36Issue(3)：71-79,9.DOI:10.3969/j.issn.2095-0411.2024.03.008
还原型谷胱甘肽对大鼠肺成纤维细胞的影响
Effects of glutathione on rat lung fibroblasts
秦晶莹 1王车礼 1孙文平 1乔海燕 1刘恒宇 1周晓鹰1
作者信息
摘要
Abstract
The purpose of this study was to investigate the effect of excessive GSH on LFS,and to ex-plore the status changes after the redox balance was broken.Fresh rat LFS were extracted and cul-tured in vitro.Cell MTT test,gelatin enzyme assay,cell morphology test,scratch test,cell immuno-fluorescence staining,and western blot were employed to observe the proliferation,growth,and mi-gration of LFS with varying concentrations of GSH as well as changes in signaling pathways.The results revealed that GSH altered intracellular homeostasis of LFS,inhibited MMP-2/9 activity,and increased the expressions of focal adhesion kinase(FAK),nuclear factor kappa-B(NF-κB),phos-pho-histone gamma H2A.X(γ-H2A.X).But the excessive GSH inhibited cell migration,p38 and p53 protein expressions.In summary,excessive GSH may induce DNA damage and exhaustion in LFS cells leading to lung problems.In addition,excessive use of antioxidants can disrupt cellular homeo-stasis and may cause irreversible damage to the body.关键词
还原型谷胱甘肽/肺纤维化/肺成纤维细胞/氧化还原平衡Key words
glutathione/pulmonary fibrosis/lung fibroblasts/redox equilibrium分类
医药卫生引用本文复制引用
秦晶莹,王车礼,孙文平,乔海燕,刘恒宇,周晓鹰..还原型谷胱甘肽对大鼠肺成纤维细胞的影响[J].常州大学学报（自然科学版）,2024,36(3):71-79,9.
