军事医学2024，Vol.48Issue(5)：347-354,8.DOI:10.7644/j.issn.1674-9960.2024.05.005
长链非编码RNA MALAT1调控星形胶质细胞增殖和凋亡及对MAPK/ERK1,2信号通路的影响
Long non-coding RNA MALAT1 regulates astrocyte proliferation and apoptosis and affects MAPK/ERK1,2 signaling pathway
摘要
Abstract
Objective To investigate the effect of MALAT1 expressions on cell proliferation and apoptosis in astrocytes by regulating mitogen-activated protein kinase(MAPK)/extracellular signal-regulated kinase(ERK1,2)pathway.Methods The MALAT1 gene was knocked down and over-expressed in C8-D1A cells by lentiviral and plasmid vectors,respectively.The expressions of MALAT1,cell proliferation-related markers(Ki67,MCM2,PCNA)and apoptosis-related proteins(Caspase-3,Bax,Bcl-2)were detected by quantitative real-time polymerase chain reaction(qPCR).CCK-8 assay and flow cytometry were used for cell proliferation and apoptosis in C8-D1A cells.Immunofluorescence was adopted to detect the protein expressions of Caspase-3 and Ki67.Western blotting was used to detect the protein expressions of Caspase-3,Bax,Bcl-2,ERK1/2,p-ERK1/2,p38MAPK and p-p38MAPK.Results Compared with the control group,over-expressed MALAT1 inhibited cell proliferation and induced cell apoptosis in C8-D1A cells while the knockdown of MALAT1 significantly enhanced cell proliferation and anti-apoptotic ability in C8-D1A cells.The proportion of C8-D1A cells in G0/G1-phase and G2/M-phase was higher than in the control group as evidenced by flow cytometry,but was lower in S-phase.Meanwhile,data showed that Caspase-3 was increased while p-ERK1/2 was decreased in terms of protein levels.The mRNA expressions of Ki67 and PCNA were decreased.After knockdown of MALAT1,the proportion of C8-D1A cells in S-phase was higher,but was lower in G2/M-phase.The protein expressions of Caspase-3 and Bax decreased while those of p-ERK1/2 and p-p38MAPK increased.The mRNA expressions of Ki67,MCM2 and PCNA were increased.The differences were all statistically significant(P＜0.05).Conclusion MALAT1 promotes astrocyte apoptosis and inhibits proliferation by regulating the MAPK/ERK1,2 signaling pathway.关键词
肺腺癌转移相关转录本1/星形胶质细胞/丝裂原活化蛋白激酶/细胞外信号调节激酶/细胞凋亡/细胞增殖Key words
MALAT1/astrocytes/mitogen-activated protein kinase/extracellular signal-regulated kinase/cell apoptosis/cell proliferation分类
医药卫生引用本文复制引用
胡辉,钱令嘉,王雪,吴雨涵,董华丰,张玲,韦爱君,谢方,赵云,孙兆炜..长链非编码RNA MALAT1调控星形胶质细胞增殖和凋亡及对MAPK/ERK1,2信号通路的影响[J].军事医学,2024,48(5):347-354,8.基金项目
国家自然科学基金项目(82271553),北京市自然科学基金项目(5222033) （82271553）
