军事医学2024，Vol.48Issue(5)：369-375,7.DOI:10.7644/j.issn.1674-9960.2024.05.008
新型自组装抗菌肽CR-16的性质及抑菌活性研究
Properties and antibacterial activity of novel self-assembled antibacterial peptide CR-16
崔亚楠 1邹鹏飞 1龚伟 1王玉丽 1高春生 1杨阳 1杨美燕1
作者信息
- 1. 军事科学院军事医学研究院,北京 100850
- 折叠
摘要
Abstract
Objective To study the properties and antimicrobial activity of the novel self-assembled antimicrobial peptide(AMP)CR-16,and to provide experimental evidence for the treatment of bacterial infections.Methods CR-16 was designed and synthesized based on the structure of antimicrobial peptides Buforin Ⅱ and LfcinB.Dynamic light scattering(DLS),transmission electron microscopy(TEM),and X-ray diffraction(XRD)were used to characterize CR-16.Based on the results of critical micelle concentration(CMC),the self-assembled properties of CR-16 were investigated using atomic force microscopy(AFM)and circular dichroism(CD).The minimum inhibitory concentration(MIC)was used to study the inhibitory effect of CR-16 while transmission electron microscopy(TEM)was adopted to observe the interactions between CR-16 and the outer membrane of bacteria.Results AMP CR-16 was prepared as self-assemblies,which were regularly spherical in shape and stable in activity.CR-16 could inhibit both the growth of Escherichia coli and,more importantly,the growth of NDM-1-producing carbapenem-resistant Escherichia coli,promising good prospects in treating infections caused by antibiotic-resistant bacteria.Conclusion CR-16 can be self-assembled and deliver antibacterial effects against Escherichia coli.关键词
抗菌肽/自组装/抗生素耐药/大肠杆菌/抑菌活性Key words
antimicrobial peptides/self-assembly/antibiotic resistance/Escherichia coli/antimicrobial activity分类
医药卫生引用本文复制引用
崔亚楠,邹鹏飞,龚伟,王玉丽,高春生,杨阳,杨美燕..新型自组装抗菌肽CR-16的性质及抑菌活性研究[J].军事医学,2024,48(5):369-375,7.
