江西农业学报2024，Vol.36Issue(4)：40-46,7.DOI:10.19386/j.cnki.jxnyxb.2024.04.007
铁皮石斛和红龙石斛杂交后代繁育技术研究
Breeding Technology of Hybrid Offspring of Dendrobium officinale and D.nestor
摘要
Abstract
The breeding technology of hybrid offspring of Dendrobium officinale and D.nestor was studied.The results showed that when D.officinale was used as female parent in hybridization,the success rate was higher,reaching 96.7%.The best breeding culture medium was HB01(containing Hyponex No.1 3.0 g)+6-BA 0.5 mg/L+NAA 0.1 mg/L.The best proliferation culture medium was HB01+BA 0.6 mg/L+NAA 0.1 mg/L+50 g/L banana.The best intensified culture medium was HB01+BA 0.3 mg/L+NAA 0.2 mg/L+50 g/L banana.The best rooting culture medium was HB01+NAA 0.1 mg/L+IBA 0.1 mg/L+0.25 g/L peptone.The better transplanting medium for the tissue culture seedlings of filial generation was shavings and the No.5 bark(0.8～1.2 cm in diameter).The seedlings at steady state can be tied with transplant medium in the open air to create tropical garden landscape.The filial generation of the two orchid had strong resistance,and were resistant to the strong light and drought.The delicate buds were suitable for choosing as explants when superior single plant of filial generation was used for propagation.The 0.1%mercuric chloride was used to sterilize the buds for 5 min,and the sprouting culture medium was HB01+6-BA 0.6 mg/L+NAA 0.1 mg/L.关键词
铁皮石斛/红龙石斛/杂交后代/组织培养/快速繁殖Key words
Dendrobium officinale/D.nestor/Hybrid offspring/Tissue culture/Rapid propagation分类
农业科技引用本文复制引用
罗剑飘,黄海英,张逸飞,沈一跃,罗青文,潘方胤,文明富,谭嘉娜,官锦燕,陈双艳,许玉蝉..铁皮石斛和红龙石斛杂交后代繁育技术研究[J].江西农业学报,2024,36(4):40-46,7.基金项目
广东省科学院建设国内一流研究机构行动专项资金项目(2018GDASCX-0926) （2018GDASCX-0926）
湛江市非资助科技攻关计划项目"铁皮石斛和红龙石斛杂交后代的繁育技术研究"(2021B01087) （2021B01087）
湛江市"领航计划"(2020LHJH006) （2020LHJH006）
2022年度湛江幼儿师范专科学校科学研究项目(ZY2022NF02). （ZY2022NF02）
