生物灾害科学2024，Vol.47Issue(2)：262-268,7.DOI:10.3969/j.issn.2095-3704.2024.02.34
一种复配型生物种衣剂防治黄瓜苗期病害的研究
A study on control of cucumber seedling diseases with a compound biological seed coating agent
刘依宁 1袁东华 1仕影 1曾文 1桑园园 1于稳欠1
作者信息
- 1. 上海万力华生物科技有限公司,上海 201611
- 折叠
摘要
Abstract
[Objective]A compound biological seed coating agent was prepared with Paenibacillus polymyxa P6 and Peptaibols to control the diseases of damping-off,root rotandrhizoctonia stem rot during cucumber seed germination and seedling stages.[Method](1)An in vitro test was conducted to screen the biocontrol strains and the concentration of Peptaibols with effective antagonistic activities against the pathogens of Pythiumap aphanidermatum,Fusarium solani and Rhizoctonia solani causing the diseases.(2)A further screening test was carried out to check if the biocontrol strains were compatible with Peptaibols used in certain concentrations;(3)A bioassay was conducted to test the effectiveness of the compound seed coating agent on seed germination of cucumber and control of the diseases.[Result]The inhibition rates to P.ahanidermatum,F.solani and R.solani were raised with the increase of Peptaibols concentration,and the inhibition rates to both P.ahanidermatum and F.solani reached 100%when the applied concentrations were at 50 mg/L and 200 mg/L,respectively,and that to R.solani reached 69.00%when the applied concentration was at 200 mg/L.The results of compatibility test showed that P6 could be compatible well with Peptaibols when the applied concentration was at 32.5 mg/L,and combining P6 and Peptaibols to form a compound seed coating agent,the inhibition rates of it to the pathogens of P.ahanidermatum,F.solani and R.solani were 90.8%,59.7%and 90.6%,respectively,which were significantly better than that of Peptaibols or P6 used alone.The results of the bioassay showed that the treatment with the compound seed coating agent containing 106 cfu/g of P6 and 32.5 mg/L of Peptaibols were superior to that of P6 or Peptaibols used alone for controlling the three seedling diseases,and the control effective rates were 38.67%,57.00%and 72.23%,respectively.[Conclusion]P6 has a broad spectrum antagonistic activity.It can be compatible well with Peptaibols to form a stable compound seed coating agent,which can effectively control the seedling diseases of cucumber.关键词
多粘类芽胞杆菌/哌珀霉素/黄瓜苗期病害/生物防治Key words
Paenibacillus polymyxa/Peptaibols/cucumber seedling disease/biocontrol分类
农业科技引用本文复制引用
刘依宁,袁东华,仕影,曾文,桑园园,于稳欠..一种复配型生物种衣剂防治黄瓜苗期病害的研究[J].生物灾害科学,2024,47(2):262-268,7.
