昆明医科大学学报2024，Vol.45Issue(5)：23-28,6.DOI:10.12259/j.issn.2095-610X.S20240504
早发性脊柱侧弯合并胸廓发育不良综合征幼猪模型肺组织学及超微结构
Histological and Ultrastructural Studies of Lung Tissues in A Piglet Model of Early Onset Scoliosis with Combination of the Thoracic Insufficiency Syndrome
摘要
Abstract
Objective To explore the histological and ultrastructural changes in lung tissues of piglets with the early onset scoliosis(EOS)with the combination of thoracic insufficiency syndrome(TIS)before and after the treatment with the growth-friendly techniques.Methods Six-week-old piglets were selected and randomly divided into the control group(n=3),the model group(n=5)and the treatment group(n=5).An EOS+TIS piglet model was constructed at 6 weeks of age.At 14 weeks of age,orthodontic treatment was performed at the same time as the tethering was released.The piglets were euthanized at 18 weeks of age,and the lung tissues were taken for HE staining and Masson staining.Pathological observations were conducted on lung parenchyma and pulmonary vascular development in the three groups.Bronchopulmonary dysplasia and pulmonary fibrosis were evaluated quantitatively with the use of following indicators:radial alveolar count(RAC),alveolar wall thickness(AWT),alveolar septal density ratio(ASDR),respiratory film thickness(RFT),average collagen fiber volume(ACFV),pulmonary capillary density(PCD),pulmonary artery wall thickness ratio(WT%),and pulmonary artery wall cross-sectional area ratio(WA%).At the same time,the ultrastructural changes in the lung tissue of piglets model were observed under the transmission electron microscope.Results Compared with the control group,pathological observation of the three piglet groups showed that the model group exhibited the bronchopulmonary dysplasia and pulmonary fibrosis,while the treatment group showed the alleviation of these manifestations.The differences in these groups of young pigs RAC,AWT,ASDR,RFT,ACFV and PCD were statistically significant(P<0.05).The differences between the WT%and WA%groups were not statistically significant(P>0.05).Under the transmission electron microscopy,the cell structure of the model group was damaged and had the poor integrity,while the cell structure of the treatment group was improved.Conclusion The lung tissue of EOS+TIS piglets shows the characteristics of bronchopulmonary dysplasia and pulmonary fibrosis;using growth-friendly technology to treat EOS+TIS piglets,its bronchopulmonary dysplasia and pulmonary fibrosis have been improved to a certain extent.关键词
早发性脊柱侧凸/胸廓发育不良综合征/支气管肺发育不良/肺组织病理学/透射电镜Key words
Early-onset scoliosis/Thoracic insufficiency syndrome/Bronchopulmonary dysplasia/Lung histopathology/Transmission electron microscopy分类
医药卫生引用本文复制引用
李啟堂,代兴飞,张清东,严荣爽,潘呈,费德锐,马裕俭,徐世鑫,张颖..早发性脊柱侧弯合并胸廓发育不良综合征幼猪模型肺组织学及超微结构[J].昆明医科大学学报,2024,45(5):23-28,6.基金项目
国家自然科学基金资助项目(82060414) （82060414）
云南省科技厅-昆明医科大学应用基础联合专项基金资助项目(02101AY070001-150) （02101AY070001-150）
云南省兴滇英才卫生人才名医专项基金资助项目(XDYCWSRC2022002) （XDYCWSRC2022002）
