昆明医科大学学报2024，Vol.45Issue(5)：37-43,7.DOI:10.12259/j.issn.2095-610X.S20240506
益生菌对脑缺血再灌注损伤大鼠Aβ表达的影响及神经元的保护作用
The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury
摘要
Abstract
Objective To explore the effect of probiotics interventionon of the expression of Aβ in rats with cerebral ischemia-reperfusion and the protective effect of neurons.Methods Male SD rats were selected as the experimental animals and randomly divided into Sham Group,Model Group,Probiotics Group and Edaravone Group.The common carotid artery was separated only to the Sham Group While a cerebral ischemia-reperfusion model was eatablished to the other groups.The degree of brain injury was evaluated using neurobehavioral scoring;The infarct area of rat brain tissue was observed using TTC staining;HE staining was used to observe the pathological and morphological changes of neurons in the CA1 and cortical regions of the hippocampus in rats;The expression of Aβ proteins was detected using immunohistochemistry experiments.Results Compared with the Sham Group,the Model Group showed an increase in neurobehavioral scores(P<0.05),an increase in cerebral infarction focus(P<0.05),neuronal damage in the hippocampal CA1 and cortical regions,and a significant upregulation of Aβ protein expression(P<0.001,P<0.05);Compared with the Model Group,the Probiotics Group and Edaravone Group showed a decrease in neurobehavioral scores(P<0.05)and a reduction in cerebral infarction focus(P<0.05).The Probiotic Group and the Edaravone Group showed the less neuronal damage in the hippocampal CA1 and cortical regions,and the Aβ protein expression levels were significantly down regulated in both groups(P<0.001,P<0.05).The improvement was more significant in Edaravone Group.Conclusion Probiotics intervention can downregulate the expression of Aβ protein,alleviate the brain tissue damage and exert neuroprotective effects in rats with cerebral ischemia-reperfusion.关键词
益生菌/脑缺血再灌注损伤/β淀粉样蛋白/依达拉奉Key words
Probiotics/Cerebral ischemia-reperfusion injury/β amyloid/Edaravone分类
医药卫生引用本文复制引用
梁国晶,冀琨,张恺纯,张玉芳,安晶,张钰鸽,文娟,任海燕..益生菌对脑缺血再灌注损伤大鼠Aβ表达的影响及神经元的保护作用[J].昆明医科大学学报,2024,45(5):37-43,7.基金项目
新疆维吾尔自治区自然科学基金资助项目(2023D01C38) （2023D01C38）
