昆明医科大学学报2024，Vol.45Issue(5)：44-48,5.DOI:10.12259/j.issn.2095-610X.S20240507
HIF-1α通过调控上皮-间充质转化对胆囊癌侵袭转移的影响
The Effect of HIF-1α on Invasion and Metastasis of Gallbladder Cancer by Regulating the Epithelial-Mesenchymal Transformation
摘要
Abstract
Objective To explore the effect of HIF-1α on the migration and invasion of gallbladder cancer cells and its related regulatory mechanism.Methods GBC-SD cells were constructed and transfected with lentivirus,and the experimental group(HIF-1α shRNA),negative control group(empty lentivirus transfection)and blank control group(untreated)were obtained respectively.The expression levels of E-Cadherin,and N-Cadherin proteins were detected by Western blotting assay,Cell scratch assay was ued to detect the migratory ability of GBC-SD cells,and Transwell invasion assay was uesd to detect the invasive ability of GBC-SD cells.Results Compared with the control group,the expression level of E-Cadherin protein in the experimental group was significantly increased(P<0.001),while the expression level of N-Cadherin protein was significantly decreased(P<0.001),and there was no remarkable difference in the expression level of E-Cadherin and N-Cadherin proteins between the control groups(P both>0.05).In the experimental group,the cell scratch healing degree was inferior to the control group(P<0.001),and the number of penetrating molds was also inferior to the control group(P<0.001),while there was no obvious otherness in the cell scratch healing degree and the number of penetrating molds between the control groups(P>0.05).Conclusion Targeted knockdown of HIF-1α can reduce the migration and invasion ability of gallbladder cancer cells,and its mechanism of action may be related to the regulation of epithelial mesenchymal transition.关键词
胆囊肿瘤/缺氧诱导因子-1α/肿瘤迁移/肿瘤侵袭/上皮-间充质转化Key words
Gallbladder neoplasms/HIF-1α/Neoplasm metastasis/Neoplasm invasiveness/Epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
李鹏,邹仁超,代龙翱,傅嵩洲,魏东,唐波..HIF-1α通过调控上皮-间充质转化对胆囊癌侵袭转移的影响[J].昆明医科大学学报,2024,45(5):44-48,5.基金项目
云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(202101AY070001-145) （202101AY070001-145）
昆明医科大学研究生创新基金资助项目(2023S080) （2023S080）
昆明医科大学第二附属医院院内科技计划基金资助项目(2021yk012) （2021yk012）
昆明医科大学第二附属医院对外合作研究基金资助项目(2022dwhz05) （2022dwhz05）
