兰州大学学报（医学版）2024，Vol.50Issue(4)：41-47,7.DOI:10.13885/j.issn.1000-2812.2024.04.007
银杏二萜内酯葡胺治疗急性脑梗死患者外周血辅助型Th17细胞亚群水平改变与临床预后的关系
Changes in Th17 cell subsets in peripheral blood of patients with acute cerebral infarction treated with ginkgo diterpene lactone meglumine and its relationship with clinical prognosis
摘要
Abstract
Objective To observe the T cell subsets in peripheral blood of patients with subacute cerebral infarction treated by Ginkgo diterpene lactone meglumine(GDLM).The effect of GDLM on T cell subsets was also observed in mice.Methods 80 patients with subacute cerebral infarction were included in Depart-ment of Neurology,The First Hospital of Lanzhou University,and were divided into two groups:the control group and the observction group.The control group received conventional treatment,and the observation group was treated with conventional treatment and GDLM.Clinical efficacy,neurological function,national institute of hea-lth stroke scores(NIHSS),level of CRP in serum,and frequency of Th17 cell subsets were compared between the two groups.In addition,C57B/L6 male mice were divided into a PBS and GDLM group.Mice were given intraperitoneal injection of PBS or GDLM and T cell subsets in spleen and draining lymph node were analyzed by FACS.Result Before treatment,there were no significant difference in NIHSS and serum CD4+T cell levels between the two groups(P>0.05).After treatment,CD4+T cell in both groups underwent no significant changes.But the level of serum CRP,frequency of CD4+IL-17A+T cells(Th17 cell)were decreased(P<0.05).Besides,NIHSS in the observation group were lower than those in the control group(P<0.05).To examine whether GDLM treatment in naïve mice alters Th17 and other T cell subsets in vivo,we intraperitoneally injected GDLM into naïve B6 male mice for 10 consecutive days.On day 5,CD4+CD44low CD62Lhigh T cells in GDLM group were significantly increased compared to that control group(P<0.05).And on day 10,CD4+CD44low CD62Lhigh T cells in GDLM group were still slightly higher.On day 5,splenic Th17 cells in GDLM group showed a decreasing trend,and were significantly lower than control group on day 10(P<0.05).Conclusion The GDLM had positive effect on subacute cerebral infarction,for which the regulation of the peripheral blood T cell subsets,especially the inhibitions of T cell activation and Th17 cell differentiation,should possibly be considered.关键词
急性脑梗死/银杏二萜内酯葡胺/Th17细胞/美国国立卫生研究院卒中量表评分/C反应蛋白Key words
acute cerebral infarction/ginkgo diterpene lactone glucamine/Th17 cell/national institute of health stroke scores/C-reactive protein分类
医药卫生引用本文复制引用
田家乐,史梅,谷有全,姚利和,魏浩广,杨军清,雒扬..银杏二萜内酯葡胺治疗急性脑梗死患者外周血辅助型Th17细胞亚群水平改变与临床预后的关系[J].兰州大学学报（医学版）,2024,50(4):41-47,7.基金项目
国家自然科学基金地区资助项目(81960293) （81960293）
甘肃省自然科学基金重点资助项目(20JR5RA3,20JR10RA671) （20JR5RA3,20JR10RA671）
中央高校基本科研业务费资助项目(lzujbky-2020-kb22) （lzujbky-2020-kb22）
甘肃省联合科研基金一般资助项目(23JRRA1495) （23JRRA1495）
兰州市城关区科技人才创新创业资助项目(2023RCCX0021) （2023RCCX0021）
甘肃省高等学校创新能力提升资助项目(2019B-003) （2019B-003）
兰州市科技局科技计划专项资助项目(2020-XG-47) （2020-XG-47）
中国博士后面上基金资助项目(2023M731460) （2023M731460）
