临床与实验病理学杂志2024，Vol.40Issue(5)：520-525,530,7.DOI:10.13315/j.cnki.cjcep.2024.05.014
PD-L1、CD163和CD8在淋巴细胞为主型乳腺原发癌及淋巴结转移癌中的表达及意义
Expression and significance of PD-L1,CD163 and CD8 in primary cancer and paired lymph node metastatic cancer of lymphocyte-predominant breast cancer
摘要
Abstract
Purpose To investigate the expression and prog-nostic value of programmed death-ligand 1(PD-L1),CD 163 and CD8 in lymphocyte-predominant breast cancer(LPBC),and to analyze the expression of PD-L1,CD 163 and CD8 in primary cancer and corresponding lymph node metastatic cancer.Meth-ods LPBC with complete pathological data were selected.Im-munohistochemical EnVision method was used to detect the ex-pression of PD-L1,CD163 and CD8 in 82 cases of primary cancer and 23 cases of corresponding lymph node metastatic cancer.Their relationships with clinicopathological features and prognosis were analyzed,and the difference of expression be-tween primary cancer and lymph node metastatic cancer.Results The positive rates of PD-L1 in tumor cell(TC)and immune cell(IC)in primary cancer were 25.61％(21/82)and 79.27％(65/82),respectively.The positive rates of TC-PD-L1 in grade Ⅱ and grade Ⅲ were 18.00％(9/50)and 37.50％(12/32),respectively.The expression of TC-PD-L1 was corre-lated with the histological grade(P＜0.05).The positive rates of IC-PD-L1 in tumor maximum diameter ≤2 cm and＞2 cm were 66.67％(18/27)and 85.45％(47/55),respectively.The positive rates of IC-PD-L1 in Ki67≤20％and＞20％were 60.00％(12/20)and 85.48％(53/62),respectively.The ex-pression of IC-PD-L1 was related to tumor maximum diameter and Ki67(P＜0.05).TC-PD-L1 expression was positively correlated with CD8+intratumoral tumor-infiltrating lymphocytes(iTILs)density(r=0.277,P＜0.05),and IC-PD-L1 expression was positively correlated with CD163(r=0.259,P＜0.05).High expression of CD163 was significantly correlated with shorter DFS(P＜0.05).Between primary cancer and lymph node metastatic cancer,the co-expression rates of TC-PD-L1 and IC-PD-L1 were 82.61％(19/23)and 47.82％(11/23),respectively,and the difference was not statistically significant(P＞0.05).There were statistically differences of CD163 expression and CD8+iTILs be-tween primary cancer and lymph node metastatic cancer(P＜0.05).Conclusion PD-L1 expression in primary cancer is as-sociated with poor clinicopathologic features,and high expression of CD163 suggests poor prognosis.There are differences in tumor immune microenvironment between LPBC primary cancer and lymph node metastatic cancer,and re-evaluation of PD-L1 of lymph node metastatic cancer may provide valuable guidance for clinical immunotherapy.关键词
乳腺肿瘤/淋巴细胞为主型乳腺癌/淋巴结转移/PD-L1/CD163Key words
breast neoplasm/lymphocyte-predominant breast cancer/lymph node metastasis/PD-L1/CD163分类
医药卫生引用本文复制引用
陈妙玲,杨月,张会芳,韩玉贞..PD-L1、CD163和CD8在淋巴细胞为主型乳腺原发癌及淋巴结转移癌中的表达及意义[J].临床与实验病理学杂志,2024,40(5):520-525,530,7.基金项目
山东省自然科学基金(ZR2010HM096) （ZR2010HM096）
