皮肤性病诊疗学杂志2024，Vol.31Issue(5)：313-318,6.DOI:10.3969/j.issn.1674-8468.2024.05.004
瘢痕疙瘩发病机制中的脂代谢相关基因:基于生物信息学分析和验证
Lipid metabolism-related genes in the pathogenesis of keloid:Based on bioinformatics anal-ysis and validation
摘要
Abstract
Objective To explore lipid metabolism-related genes that contribute to the devel-opment and progression of keloid based on bioinformatics.Methods The keloid related datasets were obtained from the GEO database,while lipid metabolism-related genes were downloaded from the Genecards database.Intersection analysis was performed to screen for common genes.GO and KEGG enrichment analyses of differentially expressed genes were performed.Significant differenti-ally expressed genes were validated using real-time fluorescence quantitative PCR(qRT-PCR)and immunohistochemistry.Results ACSS3 and PRKD1 were the two common genes related to lipid metabolism in keloid,with ACSS3 showing the most significant difference.The biological proces-ses of differentially expressed genes were mainly enriched in the biosynthesis of glycerides and the metabolism of fatty acid derivatives.KEGG pathways were mainly associated with the processes of glyceride metabolism and arachidonic acid metabolism.Immunohistochemistry and qPCR revealed high expression levels of ACSS3 in keloid tissue.Conclusion ACSS3 may play an important role in the occurrence and development of keloid by affecting lipid metabolism.关键词
瘢痕疙瘩/脂代谢/ACSS3/生物信息学Key words
keloids/lipid metabolism/ACSS3/bioinformatics引用本文复制引用
钟依秀,王琦,张江林..瘢痕疙瘩发病机制中的脂代谢相关基因:基于生物信息学分析和验证[J].皮肤性病诊疗学杂志,2024,31(5):313-318,6.基金项目
国家自然科学基金(82073019,82073018,82202851) （82073019,82073018,82202851）
深圳市科技创新委员会项目(JCYJ20210324113001005,JCYJ20210324114212035,JCYJ20220530151817038) （JCYJ20210324113001005,JCYJ20210324114212035,JCYJ20220530151817038）
南方科技大学-萱嘉联合工程实验室(Y01411853) （Y01411853）
广东省基础与应用基础研究基金(2023A1515110487) （2023A1515110487）
南方医科大学南方医院院长基金(2021B004) （2021B004）
