南京农业大学学报2024，Vol.47Issue(3)：415-423,9.DOI:10.7685/jnau.202403002
单细胞分选与测序技术在环境微生物领域的应用及前景
Application and prospect of single-cell isolation and sequencing technology in environmental microbiology
摘要
Abstract
As our understanding of environmental microbial ecosystems deepened,the demand for in-depth studies in this area continued to grow.Traditional methods such as culturomics and environmental DNA sequencing were no longer sufficient to fully understand the species and functional diversity of microbes.The single-cell isolation and sequencing technology emerged as a new paradigm in environmental microbiology,representing a hot topic and cutting-edge advancement.This single-cell technology shifted the focus of microbial research from the community level to the individual cell level,allowing for the precise isolation and sequencing of single microbial cells.This approach provided a deeper understanding of the interspecies and intraspecies diversity and functional characteristics of microbes.This review introduced the application of single-cell technology in environmental microbiology,elaborated on the key processes of single-cell isolation and genome amplification,and discussed the bioinformatic flow of the single-cell sequencing data analysis.It aimed to provide insights and inspiration for the development of single-cell technology in environmental microbiology.关键词
单细胞分选/单细胞测序/单细胞基因组/环境微生物Key words
single-cell isolation/single-cell sequencing/single-cell genomics/environmental microbiology分类
生物科学引用本文复制引用
郭晗玥,王礼岳,阮杨,徐琪程,凌宁,沈其荣..单细胞分选与测序技术在环境微生物领域的应用及前景[J].南京农业大学学报,2024,47(3):415-423,9.基金项目
国家自然科学基金项目(42307174) （42307174）
