靶向鸡糖皮质激素受体基因的microRNA预测与鉴定OA北大核心CSTPCD
Prediction and identification of microRNA targeting chicken glucocorticoid receptor gene
[目的]本文旨在预测和筛选靶向调控鸡糖皮质激素受体(glucocorticoid receptor,GR)表达的microRNA.[方法]用TargetScan、PicTar和miRDB软件分别预测靶向鸡GR 3′UTR microRNA并取交集;构建包含鸡GR 3′UTR的重组质粒及突变质粒,通过双荧光素酶试验鉴定microRNA和鸡GR 3′UTR的靶向性;用TargetScan和miRDB软件分别预测候选microRNA的靶基因,用DAVI…查看全部>>
[Objectives]This study aimed to predict and screen microRNA that targeted the expression of chicken glucocorticoid receptor(GR).[Methods]The microRNA targeting chicken GR 3′UTR was predicted by TargetScan,PicTar and miRDB software,respectively,and the overlapping microRNA was selected as candidates for further experiment.The recombinant plasmid and mutant plasmid containing chicken GR 3′UTR were constructed,and microRNA binding to chicken GR 3′UTR was verifi…查看全部>>
赵敏蝶;刘杰;赵茹茜
南京农业大学动物医学院/农业农村部动物生理生化重点实验室,江苏 南京 210095南京农业大学动物医学院/农业农村部动物生理生化重点实验室,江苏 南京 210095南京农业大学动物医学院/农业农村部动物生理生化重点实验室,江苏 南京 210095
畜牧业
鸡糖皮质激素受体microRNA靶向基因
chickenglucocorticoid receptormicroRNAtargeting gene
《南京农业大学学报》 2024 (3)
489-496,8
国家自然科学基金项目(32272962,31972638)"十四五"国家重点研发计划项目(2022YFD1300401)
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