南京农业大学学报2024，Vol.47Issue(3)：522-529,8.DOI:10.7685/jnau.202304005
海洋链球菌次级代谢产物Streptochlorin对金黄色葡萄球菌诱发乳腺细胞氧化应激的保护作用
The protective effects of Streptochlorin,marine Streptomyces sp.secondary metabolite,on oxidative stress of mammary cells induced by Staphylococcus aureus
摘要
Abstract
[Objectives]The study aimed to explore the effect and mechanism of Streptochlorin on oxidative stress of mice mammary epithelial cells induced by Staphylococcus aureus infection.[Methods]Mice mammary epithelial cells(EpH4-Ev)were pretreated with Streptochlorin,then the S.aureus(MOI=10)was added for 4 h to collect samples to clarify the effect and underlying mechanism of Streptochlorin on oxidative stress induced by S.aureus in EpH4-Fv cells,the levels of related indicators were detected by cell viability test,inverted fluorescence microscope,biochemical test,Western blot,RT-qPCR,complemented by computer molecular docking technology.[Results]20 μmol·L-1 Streptochlorin pretreatment was found to significantly ameliorate the morphological changes such as disorganization and wrinkling of EpH4-Ev cells caused by S.aureus invasion,and reduce the secretion of the cell supernatant damage markers lactate dehydrogenase(LDH)and N-acetyl-β-D-aminoglucosidase(NAGase)as well as the gene expression of classical inflammatory cytokines,such as tumor necrosis factor(TNF-α),interleukin 1β(IL-1β)and interleukin 6(IL-6)(P<0.05).Streptochlorin pretreatment significantly reduced intracellular reactive oxygen species(ROS)levels,increased total antioxidant capacity of cells(T-AOC)capacity,and decreased malondialdehyde(MDA)level in EpH4-Ev cells(P<0.05).Western blot and molecular docking results tentatively demonstrated that Streptochlorin might exert antioxidant effects through Keap1-Nrf2 signaling pathway and promote the gene expression of downstream molecules heme oxygenase 1(HO-1),NAD(P)H quinone oxidoreductase 1(NQO1),catalase(CAT),and glutathione-S-transferase(GST)(P<0.05).[Conclusions]Streptochlorin had significant protective effects on oxidative stress and inflammatory responses of EpH4-Ev cells induced by S.aureus infection,which might be associated with Keap1-Nrf2 pathway activation.关键词
海洋链球菌/Streptochlorin/金黄色葡萄球菌/氧化应激/Keap1-Nrf2Key words
marine Streptomyces sp./Streptochlorin/Staphylococcus aureus/oxidative stress/Keap1-Nrf2分类
农业科技引用本文复制引用
扶绍东,王子菁,王正磊,黄代钏,石皖荣,苗晋锋,张明智..海洋链球菌次级代谢产物Streptochlorin对金黄色葡萄球菌诱发乳腺细胞氧化应激的保护作用[J].南京农业大学学报,2024,47(3):522-529,8.基金项目
国家自然科学基金项目(22177051,32061143045) （22177051,32061143045）
中央高校基本业务费专项资金(KYCYXT2022010) （KYCYXT2022010）
四川省科技计划项目(2022YFN0068) （2022YFN0068）
