UBE2T通过JAK-STAT通路促进甲状腺乳头状癌细胞增殖OA北大核心CSTPCD

Objective:This study aims to investigate the expression of ubiquitin-binding enzyme E2T(UBE2T)in papillary thyroid carcinoma(PTC)tissues and its effect on patient prognoses.Additionally,the effects of UBE2T on PTC cell function were explored,aiming to identify potential regulatory pathways and provide theoretical foundations for future targeted therapies.Methods:By using The Cancer Genome Atlas(TCGA)database,the UBE2T expression in PTC tissues and its association with patient prognosis were systematically analyzed.The UBE2T expression in tumor tissues and adjacent normal tissues of thyroid were assessed by Western blot.UBE2T knockdown experiments were performed in PTC cell lines(TPC-1 and KTC-1).Cell proliferation,migration,and invasion abilities were evaluated by using CCK-8 and colony formation,wound healing and Transwell assays,respectively,with Western blot measuring protein levels.Results:The TCGA analysis revealed significantly elevated UBE2T expression levels in PTC tissues,correlated with disease-free interval and lymph node metastasis(P＜0.01).The UBE2T knockdown led to decreased proliferation,migration,and invasion ability in TPC-1 and KTC-1 cells,accompanied by the reduced STAT phosphorylation levels.The proliferation of UBE2T-knockdown cells significantly increased when treated with a STAT activator.Conclusion:The UBE2T knockdown suppresses the proliferation,migration,and invasion of PTC cell lines,suggesting UBE2T as a potential therapeutic target for PTC by modulating the JAK-STAT signaling pathway.