南京医科大学学报（自然科学版）2024，Vol.44Issue(6)：860-867,8.DOI:10.7655/NYDXBNSN230778
MicroRNA-21/PARP-1作为生物标志物在AR和CARAS中的诊断价值分析
Analysis of the diagnostic value of microRNA-21/PARP-1 as biomarkers in AR and CARAS
摘要
Abstract
Objective:To evaluate the diagnostic value of peripheral blood microRNA(miR)-21 and plasma poly(ADP-ribose)polymerase-l(PARP-1)in allergic rhinitis(AR)and combined allergic rhinitis and asthma syndrome(CARAS).Methods:Peripheral blood samples were collected from 44 CARAS patients,31 AR patients,and 42 healthy controls.The expression levels of miR-21 in peripheral blood were detected by RT-qPCR,and the plasma levels of PARP-1 protein were measured by ELISA.Correlation analysis was performed by rearson correlation analysis.The diagnostic sensitivity and specificity of miR-21 and PARP-1 were determined by receiver operating characteristic(ROC)curve.Results:The expression of peripheral blood miR-21 was high in CARAS patients compared with healthy controls.The level of PARP-1 was higher in AR patients than that in CARAS patients and healthy controls.Pearson correlation analysis showed that the expiression of miR-21 was correlated with eosinophils count in AR patients and with fractional nasal nitric oxide(FnNO)in CARAS patients.The plasma level of PARP-1 was correlated with forced expiratory volume in one second percent predicted(FEV1％pred)in CARAS patients and with FEV1％pred and forced expiratory volume in one second(FEV1)/forced vital capacity(FVC)(FEV1/FVC)in CARAS patients.ROC curve analysis showed that when peripheral blood miR-21 was used as a diagnostic marker for CARAS,the sensitivity was 51.35％and the specificity was 80.95％.When the plasma PARP-1 was used as a diagnostic marker for AR,the sensitivity was 90.32％and the specificity was 54.76％.When the plasma PARP-1 was used as a diagnostic marker for the progression from AR to CARAS,the sensitivity was 45.45％and the specificity was 90.32％.Conclusion:There are differential expressions of miR-21 and PARP-1 in peripheral blood of patients with AR and CARAS.the peripheral bolld miR-21 can serve as a diagnostic biomarker for CARAS,while the plasma PARP-1 can serve as a diagnostic biomarker for AR and as a biomarker for the progression from AR to CARAS.This has significant value in identifying diagnostic and therapeutic targets for AR and CARAS.关键词
过敏性鼻炎-哮喘综合征/microRNA-21/PARP-1/生物标志物Key words
combined allergic rhinitis and asthma syndrome/microRNA-21/PARP-1/biomarker分类
医药卫生引用本文复制引用
肖心儒,丁紫琪,刘志光,毛正道,张倩..MicroRNA-21/PARP-1作为生物标志物在AR和CARAS中的诊断价值分析[J].南京医科大学学报（自然科学版）,2024,44(6):860-867,8.基金项目
江苏省社会发展项目(BE2020651) （BE2020651）
江苏省"333"高层次人才项目(BRA2020015) （BRA2020015）
常州市高层次医学人才项目(2022CZLJ013) （2022CZLJ013）
南京医科大学常州医学中心科研项目(CMCB202214) （CMCB202214）
