内蒙古民族大学学报(自然科学版)2024,Vol.39Issue(3):6-13,8.DOI:10.14045/j.cnki.15-1220.2024.03.002
不同抗性大麦品种对叶斑病的生理响应及主成分分析
Physiological Responses and Principal Component Analysis of Various Resistant Barley Varieties to Leaf Spot Disease
摘要
Abstract
Four barley varieties with different resistance were used as materials to study the infection of leaves with Bipolaris sorokiniana.The activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),L-phe-nylalanine ammonia-lyase(PAL)and polyphenol oxidase(PPO)and the contents of malondialdehyde(MDA)and proline(Pro)in leaf tissues were changed.The results showed that MDA content increased first and then decreased with the extension of inoculation time,and peaked at 2.0 and 40.0 days,respectively.However,other measurements showed an increasing trend and reached a peak at 40.0 d.The activity of SOD,POD,CAT,PAL,PPO and Pro of resistant varieties were higher than those of susceptible varieties,while the MDA content of susceptible varieties was higher than that of resistant varieties.Through principal component analysis,it was found that POD activity and Pro content were significantly correlated with inoculation time,and the cumulative contribution rate of principal component was 86.27%,which could represent 86%physiological information of various barley varieties infected by Bipolaris sorokiniana.POD and Pro could be used as indicators to determine the change of enzyme activity of barley against spot blotch.关键词
大麦/叶斑病/抗病性/生理指标/主成分分析Key words
barley/leaf spot disease/disease resistance/physiological index/principal component analysis分类
农业科技引用本文复制引用
苏日娜,李建波,刘志萍,巴图,马宇,徐寿军..不同抗性大麦品种对叶斑病的生理响应及主成分分析[J].内蒙古民族大学学报(自然科学版),2024,39(3):6-13,8.基金项目
内蒙古自治区科技计划项目(2021GG0007) (2021GG0007)