农业生物技术学报2024，Vol.32Issue(6)：1250-1273,24.DOI:10.3969/j.issn.1674-7968.2024.06.003
草莓MYB基因家族的鉴定及在果实不同着色期的表达分析
Identification of MYB Gene Family in Strawberry(Fragaria vesca)and Expression Analysis at Different Coloration Stages of Fruits
摘要
Abstract
Fruit coloration is mainly determined by flavonoids such as anthocyanins,and MYB transcription factors play an important role in the regulation of anthocyanins.In order to explore the effect of strawberry(Fragaria vesca)MYB gene family on strawberry fruit coloring,in this study,114 MYB gene family members were identified by homology alignment of the MYB gene family sequences in the Arabidopsis thaliana and strawberry gene databases.The chromosomal positioning,physicochemical properties,subcellular localization and secondary structure analysis of the strawberry MYB gene family were analyzed by bioinformatics analysis software to determine the naming of the genes and the location of the main expression.The gene family was classified and its functions were predicted based on collinearity,gene structure,and promoter cis-acting element prediction.The results showed that MYB family members were predominantly expressed in the nucleus,and all but FvMYB7 were hydrophilic proteins.Among the 114 members,107 proteins exhibited interactions with each other.A relative synonymous codon usage value was greater than 2.0.This indicated that there were highly preferred codons in strawberry MYB gene.114 members could be divided into 3 subgroups according to the phylogenetic tree.Except FvMYB72 which didn't contain any action elements,all other members contained light,abiotic stress,hormone and meristem response elements.qRT-PCR analysis indicated that most members were in the S3(50%coloring period)with high relative expression levels,however,the expressions of FvMYB33,FvMYB57,and FvMYB75 were higher in the S4(full coloring stage).The tissue-specific expression profile results showed that only the FvMYB33 was specifically expressed in the fruit peel,and the results of anthocyanin content showed the highest content was in S4 period.It was speculated that FvMYB33 was the main gene regulating strawberry fruit coloring.This study provides a reference to further explore the mechanism of MYB gene family in strawberry fruit coloring.关键词
MYB基因家族/花青素/qRT-PCR/生物信息学分析Key words
MYB gene family/Anthocyanin/qRT-PCR/Bioinformatics analysis分类
农业科技引用本文复制引用
梁卫,王海,丰勇青,张澳宁,田雪纯,南昕彤,李文芳,刘飞云,马宗桓..草莓MYB基因家族的鉴定及在果实不同着色期的表达分析[J].农业生物技术学报,2024,32(6):1250-1273,24.基金项目
甘肃农业大学学生科研训练计划(SRTP)(202110733019) （SRTP）
高等学校创新基金(2021B-142) （2021B-142）
2022年青年导师基金(GAU-QDFC-2022-15) （GAU-QDFC-2022-15）
甘肃农业大学伏羲青年英才(GAUfx-04Y05) （GAUfx-04Y05）
甘肃省青年人才托举工程(GXH202220530-06) （GXH202220530-06）
