草莓MYB基因家族的鉴定及在果实不同着色期的表达分析OA北大核心CSTPCD

Fruit coloration is mainly determined by flavonoids such as anthocyanins,and MYB transcription factors play an important role in the regulation of anthocyanins.In order to explore the effect of strawberry(Fragaria vesca)MYB gene family on strawberry fruit coloring,in this study,114 MYB gene family members were identified by homology alignment of the MYB gene family sequences in the Arabidopsis thaliana and strawberry gene databases.The chromosomal positioning,physicochemical properties,subcellular localization and secondary structure analysis of the strawberry MYB gene family were analyzed by bioinformatics analysis software to determine the naming of the genes and the location of the main expression.The gene family was classified and its functions were predicted based on collinearity,gene structure,and promoter cis-acting element prediction.The results showed that MYB family members were predominantly expressed in the nucleus,and all but FvMYB7 were hydrophilic proteins.Among the 114 members,107 proteins exhibited interactions with each other.A relative synonymous codon usage value was greater than 2.0.This indicated that there were highly preferred codons in strawberry MYB gene.114 members could be divided into 3 subgroups according to the phylogenetic tree.Except FvMYB72 which didn't contain any action elements,all other members contained light,abiotic stress,hormone and meristem response elements.qRT-PCR analysis indicated that most members were in the S3(50%coloring period)with high relative expression levels,however,the expressions of FvMYB33,FvMYB57,and FvMYB75 were higher in the S4(full coloring stage).The tissue-specific expression profile results showed that only the FvMYB33 was specifically expressed in the fruit peel,and the results of anthocyanin content showed the highest content was in S4 period.It was speculated that FvMYB33 was the main gene regulating strawberry fruit coloring.This study provides a reference to further explore the mechanism of MYB gene family in strawberry fruit coloring.