农业生物技术学报2024，Vol.32Issue(6)：1307-1315,9.DOI:10.3969/j.issn.1674-7968.2024.06.007
薰衣草LibHLH75基因的克隆、表达及功能初步分析
Cloning,Expression and Preliminary Functional Analysis of LibHLH75 Gene from Lavender(Lavandula×intermedia)
摘要
Abstract
Lavender(Lavandula)is an important aromatic crop,and the differences in the quality of its essential oil are mainly caused by variations in the content of terpenoids,such as linalool and linalyl acetate.Studying the relevant genes that may be involved in regulating the biosynthesis of essential oils in lavender can provide genetic resources for breeding excellent lavender germplasm.The basic Helix-Loop-Helix(bHLH)transcription factor family plays a crucial role in plant growth,development,and secondary metabolism.In this study,the lavender LibHLH75 gene(GenBank No.OR019688)was cloned from the'Zahua'of lavender(Lavandula×intermedia),with an open reading frame of 831 bp,encoding 276 amino acids,which was closest to the bHLH75-like protein from Salvia splendens and bHLH75 protein from S.hispanica.qRT-PCR analysis showed that the LibHLH75 gene was expressed in different tissues of lavender and at different flowering stages of the corolla,with the highest expression level at the calyx and bud stages.The results of subcellular localization and transcriptional activation activity identification indicated that LibHLH75 encoded protein was localized in the nucleus,and had transcriptional activation activity.This study preliminary analyzed the function of LibHLH75,which provides a theoretical basis for further revealing the molecular regulatory mechanism of bHLH transcription factors in the biosynthesis of lavender terpenoids.关键词
'杂花'薰衣草/LibHLH75转录因子/基因克隆/表达模式/转录激活Key words
Lavandula×intermedia/LibHLH75 transcription factors/Gene cloning/Expression mode/Transcriptional activation分类
农业科技引用本文复制引用
买尔孜亚古丽·阿不来克木,张佳俊,严中建,廖燕,石梅兰,李雪龙,王爱凡,苏秀娟..薰衣草LibHLH75基因的克隆、表达及功能初步分析[J].农业生物技术学报,2024,32(6):1307-1315,9.基金项目
国家自然科学基金地区科学基金(31960452) （31960452）
新疆农业大学研究生创新项目(XJAUGRI2022037) （XJAUGRI2022037）
大学生创新项目(DXSCX2021022) （DXSCX2021022）
自治区"天山英才"培养计划青年拔尖人才项目(2023TSYCCX0017) （2023TSYCCX0017）
