青岛大学学报（医学版）2024，Vol.60Issue(2)：159-163,5.DOI:10.11712/jms.2096-5532.2024.60.076
2-Iodomelatonin上调NRF2表达对缺血性脑损伤神经保护作用
Up-regulation of NRF2 by 2-Iodomelatonin plays a neuroprotective role in ischemic brain injury
摘要
Abstract
Objective To study the neuroprotective effect of 2-Iodomelatonin as the agonist of melatonin receptor 1 in mice with middle cerebral artery occlusion by up-regulating nuclear factor erythroid 2-related factor 2(NRF2).Methods The in vivo model of ischemic brain injury in mice was constructed by middle cerebral artery occlusion(MCAO).The interventions consisted of 2-Iodomelatonin and NRF2 inhibitor(Nrf2-IN-1).The infarct volume in mouse brain was determined by triphenyl tetrazolium chlo-ride staining.Western Blot was used to measure the expression levels of NRF2 in the cerebral cortical penumbra after ischemic brain injury.The effects of 2-Iodomelatonin and Nrf2-IN-1 on the motor function of mice in the subacute stage of ischemic brain in-jury were evaluated by the modified neurological deficit score(mNSS).Results The staining showed that 2-Iodomelatonin could significantly reduce the cerebral infarct volume in mice with ischemic brain injury(F=7.79,q=5.06,P＜0.05).Western Blot showed that 2-Iodomelatonin up-regulated the expression level of NRF2 in the cerebral cortical penumbra in mice(F=10.56,q=3.99,P＜0.05).The mNSS scores showed that 2-Iodomelatonin could significantly improve the motor functions at 14 d after ische-mic brain injury(F=6.12,q=4.79,P＜0.05),and this effect could be reversed by Nrf2-IN-1(q=3.60,P＜0.05).Conclusion 2-Iodomelatonin can reduce cerebral infarct volume and improve 14-day motor function in mice with ischemic brain injury by up-re-gulating NRF2.关键词
缺血性卒中/神经保护/褪黑素/受体,褪黑激素,MT1/2-IodomelatoninKey words
ischemic stroke/neuroprotection/melatonin/receptors,melatonin,MT1/2-Iodomelatonin分类
医药卫生引用本文复制引用
高云奕,罗倩,万芪..2-Iodomelatonin上调NRF2表达对缺血性脑损伤神经保护作用[J].青岛大学学报（医学版）,2024,60(2):159-163,5.基金项目
国家自然科学基金资助项目(82071385) （82071385）
