青岛大学学报（医学版）2024，Vol.60Issue(2)：164-168,5.DOI:10.11712/jms.2096-5532.2024.60.073
TRIM45靶向IGF-1R抑制乳癌MCF-7细胞增殖和迁移的机制
Mechanism of TRIM45 targeting IGF-1R to inhibit proliferation and migration of breast cancer MCF-7 cells
摘要
Abstract
Objective To investigate the mechanism of tripartite motif-containing protein 45(TRIM45)inhibiting the pro-liferation and migration of human breast cancer MCF-7 cells by targeting insulin-like growth factor-1 receptor(IGF-1R).Methods Human breast cancer MCF-7 cells were divided into TRIM45 overexpression group,empty vector control group,and negative control group.Cell proliferation and migration abilities were determined using the cell proliferation test and scratch test.Western blot(WB)was used to measure the expression of autophagy-related protein p62,microtubule-associated protein 1 light chain 3B(LC3B),and autophagy-related protein 8(ATG8).The binding of TRIM45 with IGF-1R was analyzed by immunoprecipi-tation assay.The expression of IGF-1R and clusterin(CLU)was measured by WB.Results Compared with those in the empty vector control group,the proliferation rate and migration rate of MCF-7 cells in the TRIM45 overexpression group were significant-ly decreased(F=19.87-177.91,P＜0.05).Compared with the empty vector control group,the TRIM45 overexpression group showed significantly decreased expression of p62 and significantly increased expression of LC3B and ATG8(F=22.97-113.50,P＜0.05).TRIM45 could bind to IGF-1R.The protein expression of IGF-1R and CLU was significantly lower in the TRIM45 over-expression group than in the empty vector control group(F=51.06,30.45,P＜0.05).Conclusion TRIM45 can bind to IGF-1R to inhibit the expression of IGF-1R and CLU,inducing the autophagy of breast cancer cells to inhibit the proliferation and migration of breast cancer cells.关键词
乳腺肿瘤/三结构域蛋白45/受体,IGF 1型/细胞增殖/细胞运动Key words
breast neoplasms/tripartite motif-containing protein 45/receptor,IGF type 1/cell proliferation/cell move-ment分类
医药卫生引用本文复制引用
曹璨,董晓雷,李双,李冰..TRIM45靶向IGF-1R抑制乳癌MCF-7细胞增殖和迁移的机制[J].青岛大学学报（医学版）,2024,60(2):164-168,5.基金项目
国家自然科学基金资助项目(81871231) （81871231）
